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Highly stomach-selective gene transfer following gastric serosal surface instillation of naked plasmid DNA in rats.
http://hdl.handle.net/10069/22669
http://hdl.handle.net/10069/22669e9ad937d-9d8c-48a5-826c-fddb67cfc114
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
JouGas43_912.pdf (653.4 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||||
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| 公開日 | 2010-01-04 | |||||||||||||||||||||
| タイトル | ||||||||||||||||||||||
| タイトル | Highly stomach-selective gene transfer following gastric serosal surface instillation of naked plasmid DNA in rats. | |||||||||||||||||||||
| 言語 | ||||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | Gene delivery | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | Naked plasmid DNA | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | Rat | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | Stomach | |||||||||||||||||||||
| キーワード | ||||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||||
| 主題 | Targeting | |||||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||||
| 著者 |
Nishi, Junya
× Nishi, Junya
× Fumoto, Shintaro
× Ishii, Hiroki
× Kodama, Yukinobu
× Nakashima, Mikiro
× Sasaki, Hitoshi
× Nakamura, Junzo
× Nishida, Koyo
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| 抄録 | ||||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||||
| 内容記述 | BACKGROUND: The purpose of this study was to achieve stomach-selective gene transfer in rats by our simple and novel administration method, which is gastric serosal surface instillation of naked plasmid DNA (pDNA). METHODS: Naked pDNA encoding firefly luciferase as a reporter gene was instilled onto the gastric serosal surface in male Wistar rats. As controls, we performed intraperitoneal, intragastric and intravenous administration of naked pDNA. At appropriate time intervals, we measured luciferase activities in the stomach and other tissues. RESULTS: Gene expression in the stomach 6 h after gastric serosal surface instillation of naked pDNA (5 microg) was significantly higher than that after using other administration methods. The present study is the first report on stomach-selective gene transfer following instillation of naked pDNA onto the gastric serosal surface in rats. Also, the gene expression level in the stomach 6 h after gastric serosal surface instillation of naked pDNA was markedly higher than that in other tissues. In a dose-dependent study, the gene expression level was saturated over 5 microg. Gene expression in the stomach was detected 3 h after gastric serosal surface instillation of naked pDNA. The gene expression level peaked 12-24 h after instillation of naked pDNA, then decreased to a level similar to 3 h at 48 h. CONCLUSIONS: Gastric serosal surface in stillation of naked pDNA can be a highly stomach-selective gene transfer method in rats. | |||||||||||||||||||||
| 書誌情報 |
Journal of gastroenterology 巻 43, 号 12, p. 912-919, 発行日 2008-12 |
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| 出版者 | ||||||||||||||||||||||
| 出版者 | Springer Japan | |||||||||||||||||||||
| ISSN | ||||||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||||||
| 収録物識別子 | 09441174 | |||||||||||||||||||||
| 書誌レコードID | ||||||||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||||||||
| 収録物識別子 | AA10988015 | |||||||||||||||||||||
| PubMed番号 | ||||||||||||||||||||||
| 関連タイプ | isVersionOf | |||||||||||||||||||||
| 識別子タイプ | PMID | |||||||||||||||||||||
| 関連識別子 | 19107334 | |||||||||||||||||||||
| DOI | ||||||||||||||||||||||
| 関連タイプ | isVersionOf | |||||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||||
| 関連識別子 | 10.1007/s00535-008-2301-7 | |||||||||||||||||||||
| 権利 | ||||||||||||||||||||||
| 権利情報 | c Springer Japan 2008. | |||||||||||||||||||||
| 権利 | ||||||||||||||||||||||
| 権利情報 | The original publication is available at www.springerlink.com | |||||||||||||||||||||
| 著者版フラグ | ||||||||||||||||||||||
| 出版タイプ | AM | |||||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||||||||
| 引用 | ||||||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||||||
| 内容記述 | Journal of gastroenterology, 43(12), pp.912-919; 2008 | |||||||||||||||||||||
