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Phylogeny and Toxin Profile of Freshwater Pufferfish (Genus Pao) Collected from 2 Different Regions in Cambodia
http://hdl.handle.net/10069/40398
http://hdl.handle.net/10069/40398b7de21f2-ab02-4fb6-a988-e18d60a1c4c5
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Toxins12_689.pdf (3.2 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2020-11-19 | |||||
| タイトル | ||||||
| タイトル | Phylogeny and Toxin Profile of Freshwater Pufferfish (Genus Pao) Collected from 2 Different Regions in Cambodia | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Cambodia | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Pao | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | phylogenetic analysis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | pufferfish | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | saxitoxin (STX) | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | tributyltin-binding protein type 2 (TBT-bp2) | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Zhu, Hongchen
× Zhu, Hongchen× Yamada, Akinori× Goto, Yui× Horn, Linan× Ngy, Laymithuna× Wada, Minoru× Doi, Hiroyuki× Lee, Jong Soo× Takatani, Tomohiro× Arakawa, Osamu |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The species classification of Cambodian freshwater pufferfish is incomplete and confusing, and scientific information on their toxicity and toxin profile is limited. In the present study, to accumulate information on the phylogeny and toxin profile of freshwater pufferfish, and to contribute to food safety in Cambodia, we conducted simultaneous genetic-based phylogenetic and toxin analyses using freshwater pufferfish individuals collected from Phnom Penh and Kratie (designated PNH and KTI, respectively).Phylogenetic analysis of partial sequences of three mitochondrial genes (cytochrome b, 16S rRNA, and cytochrome c oxidase subunit I) determined for each fish revealed that PNH and KTI are different species in the genus Pao (designated Pao sp. A and Pao sp. B, respectively). A partial sequence of the nuclear tributyltin-binding protein type 2 (TBT-bp2) gene differentiated the species at the amino acid level. Instrumental analysis of the toxin profile revealed that both Pao sp. A and Pao sp. B possess saxitoxins (STXs), comprising STX as the main component. In Pao sp. A, the toxin concentration in each tissue was extremely high, far exceeding the regulatory limit for STXs set by the Codex Committee,whereas in Pao sp. B, only the skin contained high toxin concentrations. The difference in the STX accumulation ability between the two species with different TBT-bp2 sequences suggests that TBT-bp2 is involved in STX accumulation in freshwater pufferfish. |
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| 書誌情報 |
Toxins 巻 12, 号 11, p. 689, 発行日 2020-10-30 |
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| 出版者 | ||||||
| 出版者 | MDPI | |||||
| EISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 20726651 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.3390/toxins12110689 | |||||
| 権利 | ||||||
| 権利情報 | c2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open accessarticle distributed under the terms and conditions of the Creative Commons Attribution(CC BY) license (http://creativecommons.org/licenses/by/4.0/) | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Toxins, 12(11), art.no.689; 2020 | |||||
