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  1. 120 熱帯医学研究所 = Institute of Tropical Medicine
  2. 120 学術雑誌論文 = Articles in academic journal

Proteome approach for identification of schistosomiasis japonica vaccine candidate antigen.

http://hdl.handle.net/10069/21353
http://hdl.handle.net/10069/21353
949c2f6a-73a2-4c95-ad4f-4e1f25a74784
名前 / ファイル ライセンス アクション
ParInt58_36.pdf ParInt58_36.pdf (1.7 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2009-03-23
タイトル
タイトル Proteome approach for identification of schistosomiasis japonica vaccine candidate antigen.
言語
言語 eng
キーワード
主題Scheme Other
主題 Schistosoma japonicum
キーワード
主題Scheme Other
主題 Radiation-attenuated cercariae
キーワード
主題Scheme Other
主題 vaccine
キーワード
主題Scheme Other
主題 miniature pig
キーワード
主題Scheme Other
主題 proteome
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
著者 Abdel-Hafeez, Ekhlas Hamed

× Abdel-Hafeez, Ekhlas Hamed

WEKO 69163

Abdel-Hafeez, Ekhlas Hamed

Search repository
Kikuchi, Mihoko

× Kikuchi, Mihoko

WEKO 69164

Kikuchi, Mihoko

Search repository
Watanabe, Kanji

× Watanabe, Kanji

WEKO 69165

Watanabe, Kanji

Search repository
Ito, Takashi

× Ito, Takashi

WEKO 69166

Ito, Takashi

Search repository
Yu, Chuanxin

× Yu, Chuanxin

WEKO 69167

Yu, Chuanxin

Search repository
Chen, Honggen

× Chen, Honggen

WEKO 69168

Chen, Honggen

Search repository
Nara, Takeshi

× Nara, Takeshi

WEKO 69169

Nara, Takeshi

Search repository
Arakawa, Takeshi

× Arakawa, Takeshi

WEKO 69170

Arakawa, Takeshi

Search repository
Aoki, Yoshiki

× Aoki, Yoshiki

WEKO 69171

Aoki, Yoshiki

Search repository
Hirayama, Kenji

× Hirayama, Kenji

WEKO 69172

Hirayama, Kenji

Search repository
抄録
内容記述タイプ Abstract
内容記述 Experimental vaccination with radiation-attenuated cercariae (RAC) confers possible practical levels of resistance to challenge infection by humoral and by cellular mechanism. Here, we aimed to identify possible vaccine antigens by using specific IgG antibody from RAC vaccinated miniature pig. Two milligrams of soluble egg antigen (SEA) or schistosomal worm antigen preparation (SWAP) was fractionated using two dimensional liquid chromatography (proteome PF 2D) consisted of high performance chromatofocusing (HPCF) and high resolution reversed phase chromatography (HPRP). Of the 42 HPCF fractions of SEA or SWAP, 26 (61.9%) or 15 (35.7%) showed positive dot blot reaction with RAC vaccinated serum respectively. The dot blot positive fractions were applied to the second HPRP column. One hundred and seven out of 26 x 96 of SEA fractions and 18 out of 15 x 96 SWAP fractions reacted with RAC vaccinated serum. From the positive fractions we chose 17 of SEA and 10 of SWAP that had no reactivity with normal cercariae infected (NCI) sera and had single peak of 214 nm; and automated N-terminal amino acid sequence based on in situ Edman Reaction was conducted. Four sequences were obtained and applied to the homology search in NCBI database. A total of eight candidate genes were listed up and their cDNA clones from schistosomula stage were obtained. Two of the recombinant proteins (AAW27472.1 and AXX25883.1) showed strong reactivity with the RAC vaccinated serum but marginal with NCI serum. This protocol using proteome PF 2D could be applicable in identifying immunoreactive proteins from crude extract for the development of vaccines or for diagnostics.
書誌情報 Parasitology international

巻 58, 号 1, p. 36-44, 発行日 2009-03
出版者
出版者 ELSEVIER
ISSN
収録物識別子タイプ ISSN
収録物識別子 13835769
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA11536121
PubMed番号
関連タイプ isVersionOf
識別子タイプ PMID
関連識別子 18940265
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 10.1016/j.parint.2008.09.004
権利
権利情報 Copyright (c) 2008 Elsevier Ireland Ltd All rights reserved.
著者版フラグ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
引用
内容記述タイプ Other
内容記述 Parasitology International, 58(1), pp.36-44; 2009
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