| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2009-05-13 |
| タイトル |
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タイトル |
A comprehensive study on the immunological reactivity of the Hsp90 molecular chaperone. |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Kawano, Toshihiro
Kobayakawa, Takeshi
Fukuma, Yutaka
Yukitake, Hideharu
Kikuchi, Yuichiro
Shoji, Mikio
Nakayama, Koji
Mizuno, Akio
Takagi, Takashi
Nemoto, Takayuki K
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Periodontitis is a chronic infectious disease, Porphyromonas gingivalis being the most implicated pathogen. In the present study, we investigated the role of P. gingivalis HtpG (PgHtpG), a bacterial ortholog of mammalian Hsp90, in the growth of P. gingivalis and also assessed the immunological cross-reactivity of the members of the Hsp90 family. Antiserum against rat liver Hsp90 potently reacted with yeast Hsp90, called Hsc82, and also weakly with human Hsp90 (hHsp90) and human mitochondrial paralog Trap1, but did not react with PgHtpG, Escherichia coli HtpG, or human endoplasmic reticulum paralog Grp94. Moreover, among 19 monoclonal antibodies raised against hHsp90, nine cross-reacted with yeast Hsc82, and one with human Grp94, but none bound to PgHtpG or E. coli HtpG. Among them, three mAbs that strongly reacted with yeast Hsc82 recognized Asn(291)-Ile(304), a conserved region of the family protein. The polyclonal antibody raised against a peptide, Met(315)-Glu(328), of human Grp94, which corresponded to the conserved region of hHsp90, cross-reacted with hHsp90, but not with other Hsp90-family members. Thus, although mammalian Hsp90 shares some immunological reactivity with yeast Hsc82, human Grp94, and human Trap1, it is considerably distinct from its bacterial ortholog, HtpG. Disruption of the P. gingivalis htpG gene neither affected bacterial survival nor altered the sensitivity of P. gingivalis to various forms of stress. |
| 書誌情報 |
Journal of biochemistry
巻 136,
号 5,
p. 711-722,
発行日 2004-11
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| 出版者 |
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出版者 |
Nihon Seikagakkai |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0021924X |
| 書誌レコードID |
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収録物識別子タイプ |
NCID |
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収録物識別子 |
AA00694073 |
| PubMed番号 |
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関連タイプ |
isVersionOf |
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識別子タイプ |
PMID |
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関連識別子 |
15632312 |
| DOI |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
10.1093/jb/mvh178 |
| 権利 |
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権利情報 |
(c) 2004 The Japanese Biochemical Society |
| 権利 |
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権利情報 |
This is a pre-copy-editing, author-produced PDF of an article accepted for publication in "Journal of Biochemistry" following peer review. The definitive publisher-authenticated version "Journal of Biochemistry 2004 136(5):711-722" is available online at: http://jb.oxfordjournals.org/cgi/content/abstract/136/5/711 |
| 著者版フラグ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 引用 |
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内容記述タイプ |
Other |
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内容記述 |
Journal of biochemistry. 136(5), pp.711-722; 2004 |
JB_136_5_711.pdf (772.4 kB)
