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Increased Chromosome Instability and Accumulation of DNA Double-strand Breaks in Werner Syndrome Cells
http://hdl.handle.net/10069/19139
http://hdl.handle.net/10069/19139f30dc4b2-6895-443b-9e11-d7b7a00acba6
名前 / ファイル | ライセンス | アクション |
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RR48_3_219_231.pdf (1.9 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-08-12 | |||||
タイトル | ||||||
タイトル | Increased Chromosome Instability and Accumulation of DNA Double-strand Breaks in Werner Syndrome Cells | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Chromosome Instability | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA Double-strand Breaks | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Werner Syndrome | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Premature senescence | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Oxidative stress | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Genomic instability | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Ariyoshi, Kentaro
× Ariyoshi, Kentaro× Suzuki, Keiji× Goto, Makoto× Watanabe, Masami× Kodama, Seiji |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Werner syndrome (WS) is a premature aging syndrome caused by mutations of the WRN gene. Here, we demonstrate that a strain of WS fibroblast cells shows abnormal karyotypes characterized by several complex translocations and 50-fold more frequency of abnormal metaphases including dicentric chromosomes without fragments than normal cells when examined at a similar culture stage. Further, telomere fluorescence in situ hybridization indicates that the abnormal signals, extra telomere signal and loss of telomere signal, emerge two- to three-fold more frequently in WS cells than in normal cells. Taken together, these results indicate that chromosome instability including dysfunction of telomere maintenance is more prominent in WS cells than in normal cells. In addition, the accumulation of DNA double-strand breaks (DSBs) at the G_1 phase, including those at telomeres, detected by phosphorylated ATM (ataxia telangiectasia mutated) foci is accelerated in WS cells even at a low senescence level. The increased accumulation of DSBs in WS cells is reduced in the presence of anti-oxidative agents, suggesting that enhanced oxidative stress in WS cells is involved in accelerated accumulation of DSBs. These results indicate that WS cells are prone to accumulate DSBs spontaneously due to a defect of WRN, which leads to increased chromosome instability that could activate checkpoints, resulting in accelerated senescence. | |||||
書誌情報 |
Journal of radiation research 巻 48, 号 3, p. 219-231, 発行日 2007-05-15 |
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出版者 | ||||||
出版者 | 日本放射線影響学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 04493060 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00705792 | |||||
権利 | ||||||
権利情報 | 日本放射線影響学会 | |||||
権利 | ||||||
権利情報 | 本文データは学協会の許諾に基づきCiNiiから複製したものである | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of radiation research. 2007, 48(3), p.219-231 |