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Cell adhesion markedly increases lucigenin-enhanced chemiluminescence of the phagocyte NADPH oxidase.
http://hdl.handle.net/10069/22029
http://hdl.handle.net/10069/22029ebe3a7cb-15c9-4e3d-8d5f-e3ea39d0c0de
名前 / ファイル | ライセンス | アクション |
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GenCel13_1249.pdf (419.4 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-07-21 | |||||
タイトル | ||||||
タイトル | Cell adhesion markedly increases lucigenin-enhanced chemiluminescence of the phagocyte NADPH oxidase. | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kuribayashi, Futoshi
× Kuribayashi, Futoshi× Tsuruta, Satoru× Yamazaki, Tsuyoshi× Nunoi, Hiroyuki× Imajoh-Ohmi, Shinobu× Kanegasaki, Shiro× Nakamura, Michio |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Lucigenin-enhanced chemiluminescence (LECL) is widely used for the detection of reactive oxygen species released from various cells and mitochondria. However, the LECL response varies depending on cell species and assay conditions at least in part by unknown factors. Here we report that cell adhesion is an important factor for increasing LECL of tetradecanoylphorbol acetate (TPA)-stimulated human neutrophils. More than 90% LECL remained even after complete removal of the cell suspension 10 min after TPA stimulation, and ~22.5% of neutrophils were adhered to the reaction tube. These results indicate that LECL by an adhering neutrophil is ~45x higher than that by a non-adhering neutrophil. LECL by leukocyte adhesion deficiency neutrophils was one-fifth of that by normal neutrophils and completely disappeared when the cell suspension was removed, confirming that LECL depends highly on cell adhesion. The oxidase activity of adhering neutrophils measured after permeabilization with Renex 30 together with NADPH addition was similar to that of non-adhering neutrophils, indicating that lucigenin and cell adhesion do not enhance the oxidase activity. Based on these findings, we propose that a mixture of adhering and non-adhering neutrophils can be used for simultaneous screenings of adhering activity and the oxidase activity of neutrophils. | |||||
書誌情報 |
Genes to cells : devoted to molecular & cellular mechanisms 巻 13, 号 12, p. 1249-1256, 発行日 2008-12 |
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出版者 | ||||||
出版者 | Blackwell Publishing Ltd | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13652443 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11078945 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 19032347 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1111/j.1365-2443.2008.01241.x | |||||
権利 | ||||||
権利情報 | c 2008 by the Molecular Biology Society of Japan/Blackwell Publishing Ltd | |||||
権利 | ||||||
権利情報 | The definitive version is available at www3.interscience.wiley.com | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Genes to Cells, 13(12), pp.1249-1256; 2008 |