WEKO3
アイテム
{"_buckets": {"deposit": "0bfb25d0-3990-47da-850b-cb9a800e8674"}, "_deposit": {"created_by": 2, "id": "19369", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "19369"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00019369", "sets": ["74"]}, "author_link": ["80650", "80651", "80649", "80648", "80647", "80652"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2008-04", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "4", "bibliographicPageEnd": "475", "bibliographicPageStart": "471", "bibliographicVolumeNumber": "24", "bibliographic_titles": [{"bibliographic_title": "Analytical sciences"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Telomerase shows increased activity in most human cancers and germ line cells, but not in normal human somatic cells. We describe a novel chemiluminescence method for the facile assay of telomerase activity in human cells. The telomerase\nsubstrate was incubated with the cell lysate containing various amounts of telomerase, and then the telomerase product was amplified by the polymerase-chained reaction (PCR). The PCR products were separated from the excess substrate, primer and deoxyribonucleotide triphosphates by a centrifugal filter, which distinguished different molecular sizes. The isolated products were reacted with a DNA-detectable chemiluminogenic reagent, 3,4,5-trimethoxyphenylglyoxal. The proposed assay method gave linearity for the telomerase activity in 100 to 10000 cells (r2 = 0.997), and allowed the assay not only of lower activity, but also of higher activity of telomerase without the requirement of any special labeled-PCR primers in the assay system.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "Analytical sciences, 24(4), pp.471-475; 2008", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "The Japan Society of Analytical Chemistry"}]}, "item_2_relation_11": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "18403837", "subitem_relation_type_select": "PMID"}}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.2116/analsci.24.471", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "Copyright (c) 2008 by The Japan Society for Analytical Chemistry"}]}, "item_2_source_id_10": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA10500785", "subitem_source_identifier_type": "NCID"}]}, "item_2_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "09106340", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Tonooka, Keiko"}], "nameIdentifiers": [{"nameIdentifier": "80647", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kabashima, Tsutomu"}], "nameIdentifiers": [{"nameIdentifier": "80648", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shibata, Takayuki"}], "nameIdentifiers": [{"nameIdentifier": "80649", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tang, Chenhong"}], "nameIdentifiers": [{"nameIdentifier": "80650", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yu, Zhiqiang"}], "nameIdentifiers": [{"nameIdentifier": "80651", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kai, Masaaki"}], "nameIdentifiers": [{"nameIdentifier": "80652", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-23"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "AnaSci24_4.pdf", "filesize": [{"value": "829.4 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 829400.0, "url": {"label": "AnaSci24_4.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/19369/files/AnaSci24_4.pdf"}, "version_id": "3901407e-51fd-410d-bc93-9021cf65ae24"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Facile assay of telomerase activity utilizing a DNA-detectable chemiluminogenic reagent.", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Facile assay of telomerase activity utilizing a DNA-detectable chemiluminogenic reagent."}]}, "item_type_id": "2", "owner": "2", "path": ["74"], "permalink_uri": "http://hdl.handle.net/10069/18702", "pubdate": {"attribute_name": "公開日", "attribute_value": "2008-07-09"}, "publish_date": "2008-07-09", "publish_status": "0", "recid": "19369", "relation": {}, "relation_version_is_last": true, "title": ["Facile assay of telomerase activity utilizing a DNA-detectable chemiluminogenic reagent."], "weko_shared_id": -1}
Facile assay of telomerase activity utilizing a DNA-detectable chemiluminogenic reagent.
http://hdl.handle.net/10069/18702
http://hdl.handle.net/10069/18702f27caafb-66bf-414f-a405-34a5edfed6ae
名前 / ファイル | ライセンス | アクション |
---|---|---|
AnaSci24_4.pdf (829.4 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2008-07-09 | |||||
タイトル | ||||||
タイトル | Facile assay of telomerase activity utilizing a DNA-detectable chemiluminogenic reagent. | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Tonooka, Keiko
× Tonooka, Keiko× Kabashima, Tsutomu× Shibata, Takayuki× Tang, Chenhong× Yu, Zhiqiang× Kai, Masaaki |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Telomerase shows increased activity in most human cancers and germ line cells, but not in normal human somatic cells. We describe a novel chemiluminescence method for the facile assay of telomerase activity in human cells. The telomerase substrate was incubated with the cell lysate containing various amounts of telomerase, and then the telomerase product was amplified by the polymerase-chained reaction (PCR). The PCR products were separated from the excess substrate, primer and deoxyribonucleotide triphosphates by a centrifugal filter, which distinguished different molecular sizes. The isolated products were reacted with a DNA-detectable chemiluminogenic reagent, 3,4,5-trimethoxyphenylglyoxal. The proposed assay method gave linearity for the telomerase activity in 100 to 10000 cells (r2 = 0.997), and allowed the assay not only of lower activity, but also of higher activity of telomerase without the requirement of any special labeled-PCR primers in the assay system. |
|||||
書誌情報 |
Analytical sciences 巻 24, 号 4, p. 471-475, 発行日 2008-04 |
|||||
出版者 | ||||||
出版者 | The Japan Society of Analytical Chemistry | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09106340 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10500785 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | 18403837 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.2116/analsci.24.471 | |||||
権利 | ||||||
権利情報 | Copyright (c) 2008 by The Japan Society for Analytical Chemistry | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Analytical sciences, 24(4), pp.471-475; 2008 |