| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2024-11-08 |
| タイトル |
|
|
タイトル |
Presence of Plasmodium falciparum strains with artemisinin-resistant K13 mutation C469Y in Busia County, Western Kenya |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Makau, Mark
Kanoi, Bernard N.
Mgawe, Calvin
Maina, Michael
Bitshi, Mimie
Too, Edwin K.
Naruse, Taeko K.
Abkallo, Hussein M.
Waweru, Harrison
Adung’o, Ferdinand
Kaneko, Osamu
Gitaka, Jesse
|
| 抄録 |
|
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内容記述タイプ |
Other |
|
内容記述 |
Malaria remains a key health and economic problem, particularly in sub-Saharan Africa. The emergence of artemisinin drug resistance (ART-R) parasite strains poses a serious threat to the control and elimination of this scourge. This is because artemisinin-based combination therapies (ACTs) remain the first-line treatment in the majority of malaria-endemic regions in Sub-Saharan Africa. Certain single-nucleotide polymorphisms in the propeller domains of Plasmodium falciparum Kelch 13 protein (K13) have been associated with delayed parasite clearance in vivo and in vitro. These mutations serve as vital molecular markers for tracking the emergence and dispersion of resistance. Recently, there have been increasing reports of the emergence and spread of P. falciparum ART-R parasites in the Eastern Africa region. This necessitates continued surveillance to best inform mitigation efforts. This study investigated the presence of all reported mutations of K13 propeller domains in the parasite population in Busia County, Kenya, a known malaria-endemic region. Two hundred twenty-six participants with microscopically confirmed uncomplicated malaria were recruited for this study. They were treated with artemether–lumefantrine under observation for the first dose, and microscopic examination was repeated 1 day later after ensuring the participants had taken the second and third doses. P. falciparum DNA from all samples underwent targeted amplification of the K13 gene using a semi-nested PCR approach, followed by Sanger sequencing. The recently validated ART-R K13 mutation C469Y was identified in three samples. These three samples were among 63 samples with a low reduction in parasitemia on day 1, suggesting day 1 parasitemia reduction rate is a useful parameter to enrich the ART-R parasites for further analysis. Our findings highlight the need for continuous surveillance of ART-R in western Kenya and the region to determine the spread of ART-R and inform containment. |
|
言語 |
en |
| bibliographic_information |
en : Tropical Medicine and Health
巻 52,
号 1,
p. art. no. 72,
発行日 2024-10-18
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| 出版者 |
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|
出版者 |
BioMed Central Ltd |
|
言語 |
en |
| item_2_source_id_7 |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
1349-4147 |
| item_2_relation_12 |
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|
関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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|
関連識別子 |
10.1186/s41182-024-00640-1 |
| 権利 |
|
|
言語 |
en |
|
権利情報 |
© The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| item_2_description_63 |
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|
内容記述タイプ |
Other |
|
内容記述 |
Tropical Medicine and Health, 52(1), art. no. 72; 2024 |
|
言語 |
en |
TMH52_72.pdf (1.5 MB)
