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TNF-α Induces Caspase-1 Activation Independently of Simultaneously Induced NLRP3 in 3T3-L1 Cells
http://hdl.handle.net/10069/37247
http://hdl.handle.net/10069/372475f6c8cbd-a18c-4136-b3b6-1945449efc7b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
JCP231_2761.pdf (362.5 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2017-08-23 | |||||
| タイトル | ||||||
| タイトル | TNF-α Induces Caspase-1 Activation Independently of Simultaneously Induced NLRP3 in 3T3-L1 Cells | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | caspase-1 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | inflammasome | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | NLRP3 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | TNF-α | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | adipocytes | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Furuoka, Mana
× Furuoka, Mana× Ozaki, Kei-Ichi× Sadatomi, Daichi× Mamiya, Sayaka× Yonezawa, Tomo× Tanimura, Susumu× Takeda, Kohsuke |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The intracellular cysteine protease caspase-1 is critically involved in obesity-induced inflammation in adipose tissue. A substantial body of evidence from immune cells, such as macrophages, has shown that caspase-1 activation depends largely on a protein complex, called the NLRP3 inflammasome, which consists of the NOD-like receptor (NLR) family protein NLRP3, the adaptor protein ASC, and caspase-1 itself. However, it is not fully understood how caspase-1 activation is regulated within adipocytes upon inflammatory stimuli. In this study, we show that TNF-α-induced activation of caspase-1 is accompanied by robust induction of NLRP3 in 3T3-L1 adipocytes but that caspase-1 activation may not depend on the NLRP3 inflammasome. Treatment of 3T3-L1 cells with TNF-α induced mRNA expression and activation of caspase-1. Although the basal expression of NLRP3 and ASC was undetectable in unstimulated cells, TNF-α strongly induced NLRP3 expression but did not induce ASC expression. Interestingly, inhibitors of the ERK MAP kinase pathway strongly suppressed NLRP3 expression but did not suppress the expression and activation of caspase-1 induced by TNF-α, suggesting that NLRP3 is dispensable for TNF-α-induced caspase-1 activation. Moreover, we did not detect the basal and TNF-α-induced expression of other NLR proteins (NLRP1a, NLRP1b, and NLRC4), which do not necessarily require ASC for caspase-1 activation. These results suggest that TNF-α induces caspase-1 activation in an inflammasome-independent manner in 3T3-L1 cells and that the ERK-dependent expression of NLRP3 may play a role independently of its canonical role as a component of inflammasomes. | |||||
| 書誌情報 |
Journal of Cellular Physiology 巻 231, 号 12, p. 2761-2767, 発行日 2016-08-22 |
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| 出版者 | ||||||
| 出版者 | Wiley Periodicals, Inc. | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00219541 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1002/jcp.25385 | |||||
| 権利 | ||||||
| 権利情報 | c 2016 Wiley Periodicals, Inc. | |||||
| 権利 | ||||||
| 権利情報 | This is the peer reviewed version of the following article: Journal of Cellular Physiology, 231(12), pp.2761-2767; 2016, which has been published in final form at http://dx.doi.org/10.1002/jcp.25385. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Journal of Cellular Physiology, 231(12), pp.2761-2767; 2016 | |||||
