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Electrophysiological Characterization of Tight Junctional Pathway of Rabbit Cornea Treated with Ophthalmic Ingredients
http://hdl.handle.net/10069/15103
http://hdl.handle.net/10069/15103a10e12bf-24ac-452d-a14d-bc9134a1fa13
| 名前 / ファイル | ライセンス | アクション |
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bpb_30_2360.pdf (404.9 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||
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| 公開日 | 2008-03-05 | |||||||||||||||||||
| タイトル | ||||||||||||||||||||
| タイトル | Electrophysiological Characterization of Tight Junctional Pathway of Rabbit Cornea Treated with Ophthalmic Ingredients | |||||||||||||||||||
| 言語 | ||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| 主題 | eye | |||||||||||||||||||
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| 主題Scheme | Other | |||||||||||||||||||
| 主題 | cornea | |||||||||||||||||||
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| 主題Scheme | Other | |||||||||||||||||||
| 主題 | permeability | |||||||||||||||||||
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| 主題Scheme | Other | |||||||||||||||||||
| 主題 | electrophysiolosy | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| 主題 | tight junction | |||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||
| 著者 |
Nakamura, Tadahiro
× Nakamura, Tadahiro
× Yamada, Mikiko
× Teshima, Mugen
× Nakashima, Mikiro
× To, Hideto
× Ichikawa, Nobuhiro
× Sasaki, Hitoshi
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| 抄録 | ||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||
| 内容記述 | The purpose of this study was to investigate the continuous and real-time influence of ophthalmic ingredients on rabbit cornea by monitoring electrophysiological characteristics. The tight junctional permeabilities of FITC-dextran 4400 (FD-4) was also determined through the cornea in the presence of ophthalmic ingredients. Intact cornea showed approximately one k-ohm×cm2 of transepithelial electrical resistance (TEER) and extremely low permeability of FD-4. The ophthalmic ingredients used in the present study were benzalkonium chloride (BK; 0.002%, 0.01%, 0.05%), ethylenediaminetetraacetic acid (EDTA; 0.5%), capric acid (C10; 0.25%), saponin (SP; 0.1%), taurocholic acid (TA; 1.0%) and sodium dodecyl sulfate (SDS; 0.01%). They were previously reported to be effective on corneal penetrations of various drugs at those concentrations without severe toxicity. These ingredients decreased TEER and increased corneal permeability of FD-4. BK reduced TEER in a concentration-dependent manner. There was a significant correlation (γ=0.860) between the permeability coefficient (Papp) of FD-4 and conductance (Gm), which is the reciprocal value of TEER. It was also indicated that Papp and Gm have a relationship with the corneal cytotoxicity of the ingredients. In conclusion, an electrophysiological method using isolated cornea was very useful to determine the continuous and real-time influence of ophthalmic ingredients on the cornea. In this method, electrophysiological conductance must be able to predict corneal tight junction permeability and the corneal cytotoxicity of ingredients. | |||||||||||||||||||
| 書誌情報 |
Biological & Pharmaceutical Bulletin 巻 30, 号 12, p. 2360-2364, 発行日 2007-12 |
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| 出版者 | ||||||||||||||||||||
| 出版者 | The Pharmaceutical Society of Japan | |||||||||||||||||||
| ISSN | ||||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||||
| 収録物識別子 | 09186158 | |||||||||||||||||||
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| 収録物識別子タイプ | ISSN | |||||||||||||||||||
| 収録物識別子 | 1347-5215 | |||||||||||||||||||
| 書誌レコードID | ||||||||||||||||||||
| 収録物識別子タイプ | NCID | |||||||||||||||||||
| 収録物識別子 | AA00602100 | |||||||||||||||||||
| PubMed番号 | ||||||||||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||||||||||
| 識別子タイプ | PMID | |||||||||||||||||||
| 関連識別子 | 18057726 | |||||||||||||||||||
| DOI | ||||||||||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||
| 関連識別子 | 10.1248/bpb.30.2360 | |||||||||||||||||||
| 著者版フラグ | ||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||
| 関係URI | ||||||||||||||||||||
| 関連名称 | http://dx.doi.org/10.1248/bpb.30.2360 | |||||||||||||||||||
| 引用 | ||||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||||
| 内容記述 | Biological & Pharmaceutical Bulletin v.30(12) p.2360-2364, 2007 | |||||||||||||||||||
