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Transport of Timolol and Tilisolol in Rabbit Corneal Epithelium
http://hdl.handle.net/10069/8395
http://hdl.handle.net/10069/8395955a0b3c-bfc0-4e13-8baa-e12aeef7ce33
名前 / ファイル | ライセンス | アクション |
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BPBul29_2143.pdf (127.0 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2007-10-12 | |||||
タイトル | ||||||
タイトル | Transport of Timolol and Tilisolol in Rabbit Corneal Epithelium | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | cultured rabbit cornea | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | drug delivery system | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | transporter | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | beta-blocker | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | tilisolol | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | timolol | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Sakanaka, Koji
× Sakanaka, Koji× Kawazu, Kouichi× Nishida, Koyo× Nakamura, Junzo× Nakashima, Mikiro× Nakamura, Tadahiro× Oshita, Akemi× Ichikawa, Nobuhiro× Sasaki, Hitoshi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The purpose of this study is to characterize the transport of tilisolol and timolol through the corneal epithelium, which is believed to be a tight barrier of ocular drug absorption. Cultured normal rabbit corneal epithelial cells (RCEC) were used to investigate drug transport. Primary RCEC were seeded on a filter membrane of Transwell-COL? insert coated with fibronectin and grown in Dulbecco's modified Eagle's medium/nutrient mixture F-12 with various supplements. Beta-blocker permeability through the RCEC layer was measured to assess the transcellular permeability coefficient (Ptranscell) in the absence or presence of inhibitors. The transcellular permeability of tilisolol was dependent on drug concentration although timolol showed no concentration dependency. Tilisolol flux from the apical to the basal side was larger than in the opposite direction although timolol showed no direction dependency. The transcellular permeability of tilisolol from the apical to the basal side was inhibited by sodium azide, tetraethylammonium, quinidine, taurocholic acid, guanidine and carnitine. Tilisolol had an active mechanism in uptake to the corneal epithelium, probably by the organic cation transporter family, although timolol predominantly permeated via passive diffusion. This RCEC system was useful to characterize the ocular permeation mechanism of drugs. | |||||
書誌情報 |
Biological & Pharmaceutical Bulletin 巻 29, 号 10, p. 2143-2147, 発行日 2006-12 |
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出版者 | ||||||
出版者 | Pharmaceutical Society of Japan | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09186158 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1347-5215 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10885497 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | 17015968 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1248/bpb.29.2143 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関係URI | ||||||
関連名称 | http://dx.doi.org/10.1248/bpb.29.2143 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biological & Pharmaceutical Bulletin v.29(10) p.2143-2147, 2006 |