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Characterization of transgene expression and pDNA distribution of the suctioned kidney in mice
http://hdl.handle.net/10069/37721
http://hdl.handle.net/10069/37721db6b8f7e-0de5-4dd8-80c1-ca7a6139816d
名前 / ファイル | ライセンス | アクション |
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DD24_906.pdf (2.1 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-08-09 | |||||
タイトル | ||||||
タイトル | Characterization of transgene expression and pDNA distribution of the suctioned kidney in mice | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Gene delivery | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | kidney | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | naked pDNA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | spatial distribution | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | fibroblast | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Oyama, Natsuko
× Oyama, Natsuko× Fuchigami, Yuki× Fumoto, Shintaro× Sato, Megumu× Hagimori, Masayori× Shimizu, Kazunori× Kawakami, Shigeru |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We have previously developed an efficient and safe transfection method for the kidney in mice: renal suction-mediated transfection. In this study, we verified the detailed characteristics of transgene expression and plasmid DNA (pDNA) in mice to develop therapeutic strategies and application to gene function analysis in the kidney. After naked pDNA was administered intravenously, the right kidney was immediately suctioned by a tissue suction device. We examined the spatial distribution of transgene expression and pDNA in the suctioned kidney using tissue clearing by CUBIC, ClearT2, and Scale SQ reagents. Spatial distribution analysis showed that pDNA was transfected into extravascular cells and sufficiently delivered to the deep renal cortex. In addition, we revealed that transgene expression occurred mainly in peritubular fibroblasts of the suctioned kidney by tissue clearing and immunohistochemistry. Next, we confirmed the periods of pDNA uptake and activation of transcription factors nuclear factor-κB and activator protein 1 by luciferase assays. Moreover, the use of a pCpG-free plasmid enabled sustained transgene expression in the suctioned kidney. In conclusion, analyses of the spatial distribution and immunostaining of the section suggest that pDNA and transgene expression occurs mainly in peritubular fibroblasts of the suctioned kidney. In addition, we clarified some factors for efficient and/or sustained transgene expression in the suctioned kidney. | |||||
書誌情報 |
Drug Delivery 巻 24, 号 1, p. 906-917, 発行日 2017-06-06 |
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出版者 | ||||||
出版者 | Informa UK Limited | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 10717544 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 15210464 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1080/10717544.2017.1333171 | |||||
権利 | ||||||
権利情報 | c2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. | |||||
権利 | ||||||
権利情報 | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Drug Delivery, 24(1), pp.906-917; 2017 |