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Use of the Reverse Transcriptase Polymerase Chain Reaction for Diagnosis on Dengue Virus Infection Compared to IgM-ELISA
http://hdl.handle.net/10069/4675
http://hdl.handle.net/10069/467566cc51ea-4817-4089-b768-aad095cd3d7b
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
tm36_03_01_t.pdf (769.0 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2006-04-26 | |||||
| タイトル | ||||||
| タイトル | Use of the Reverse Transcriptase Polymerase Chain Reaction for Diagnosis on Dengue Virus Infection Compared to IgM-ELISA | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
Saat, Zainah
× Saat, Zainah× Thayan, Ravindran× Balasubramaniam, Vijayamalar× Morita, Kouichi× Tsuchie, Hideaki× Sinniah, Mangalam× Igarashi, Akira× Tanaka, Hiroshi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Applicability of the reverse transcriptase polymerase chain reaction (RT-PCR) was evaluated as a routine rapid diagnostic test for dengue virus infection. A total of 160 acute phase sera from patients with clinical diagnosis of dengue fever was examined both by the RT-PCR and IgM-ELISA. Of these, 9 (6%) were positive for both RT-PCR and IgM-ELISA, 61 were positive for IgM-ELISA only and 31 (19%) for RT-PCR only. Both techniques gave negative results in the remaining 59 (37%) specimens. The diagnostic efficiency of IgM-ELISA was statistically better than the RT-PCR even when the specimens were collected on the 3rd or 5th days of the disease. Considering the operational cost in the tests, the acute serum specimens should first be tested by the IgM-ELISA, followed by the RT-PCR on the negative specimens in order to increase the diagnostic efficiency with reasonable cost. | |||||
| 書誌情報 |
熱帯医学 Tropical medicine 巻 36, 号 3, p. 75-81, 発行日 1994-12-28 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 03855643 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00199644 | |||||
| 出版者 | ||||||
| 出版者 | 長崎大学熱帯医学研究所 | |||||
| 出版者別言語 | ||||||
| 値 | Institute of Tropical Medicine, Nagasaki University | |||||
| sortkey | ||||||
| 値 | P00075-P00081 | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 熱帯医学 Tropical medicine 36(3). p75-81, 1994 | |||||
