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Dielectrophoresis Concentration Method for Increased Sensitivity of the Loop-Mediated Isothermal Amplification Test for the Mycobacterium tuberculosis Complex
http://hdl.handle.net/10069/00040763
http://hdl.handle.net/10069/000407639b43fc97-260e-40e3-8549-b6afd7041ebc
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ISYK1344_Takeda.pdf (3.8 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||
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| 公開日 | 2021-05-19 | |||||||
| タイトル | ||||||||
| タイトル | Dielectrophoresis Concentration Method for Increased Sensitivity of the Loop-Mediated Isothermal Amplification Test for the Mycobacterium tuberculosis Complex | |||||||
| 言語 | en | |||||||
| 言語 | ||||||||
| 言語 | eng | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Mycobacterium Tuberculosis Complex | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Dielectrophoresis | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Concentration | |||||||
| キーワード | ||||||||
| 主題Scheme | Other | |||||||
| 主題 | Nucleic Acid Amplification Test | |||||||
| 資源タイプ | ||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||||
| 資源タイプ | doctoral thesis | |||||||
| アクセス権 | ||||||||
| アクセス権 | open access | |||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||
| 著者 |
武田, 啓太
× 武田, 啓太
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| 著者別名 | ||||||||
| 識別子Scheme | WEKO | |||||||
| 識別子 | 117954 | |||||||
| 姓名 | Takeda, Keita | |||||||
| 抄録 | ||||||||
| 内容記述タイプ | Abstract | |||||||
| 内容記述 | Background: An effective concentration method is required to detect Mycobacterium tuberculosis (MTB) in paucibacillary specimens. Dielectrophoresis (DEP), a phenomenon in which a force is exerted on a dielectric particle subjected to a non-uniform electric field, is useful for concentrating bacterial specimens. Objectives: To investigate whether the procedure using the DEP method increases nucleic acid amplification test (NAAT) sensitivity. Methods: First, the capture rates were examined for multiple electrode settings by calculating the bacterial load before and after DEP using quantitative real-time PCR. These results were used to determine the optimal electrode setting. Second, conventional loop-mediated isothermal amplification (LAMP) was performed using 30 μL DNA purified using a PURE DNA extraction kit from 60 μL Mycobacterium bovis bacille de Calmette et Guérin culture fluid. The minimally-diluted specimen (theoretical concentration, 103 cfu/mL), which showed 10 consecutive negative results by LAMP, was subjected to DEP. One milliliter of the specimen was concentrated to 50 μL in the DEP chip, and LAMP was performed in 30 μL of the specimens without DNA purification. Results: At 73.2-84.9%, the capture rate was the highest in the setting with 100 kHz frequency. LAMP with DEP was performed using conventional LAMPnegative specimens, and eight of ten tests (80%) were positive. The sensitivity was higher than that of the conventional LAMP method (p = 0.0007). Conclusion: The DEP method has the potential to increase NAAT sensitivity for the MTB complex in paucibacillary specimens. |
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| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | 長崎大学学位論文 学位記番号:博(医歯薬)甲第1344号 学位授与年月日:令和3年3月22日 | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | Author: Takeda K, Chikamatsu K, Aono A, Igarashi Y, Morishige Y, Murase Y, Takaki A, Yamada H and Mitarai S | |||||||
| 内容記述 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | Citation: Journal of Bacteriology and Mycology, 7(8), id1157; 2020 | |||||||
| 書誌情報 |
Journal of Bacteriology and Mycology 巻 7, 号 8, p. 1157, 発行日 2021-03-22 |
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| ISSN | ||||||||
| 収録物識別子タイプ | ISSN | |||||||
| 収録物識別子 | 2471-0172 | |||||||
| 権利 | ||||||||
| 権利情報 | Takeda et al. © All rights are reserved | |||||||
| 著者版フラグ | ||||||||
| 出版タイプ | VoR | |||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||
| その他のタイトル | ||||||||
| その他のタイトル | 誘電泳動法を用いた結核菌濃縮法によるLoop-Mediated Isothermal Amplification法の検査感度上昇 | |||||||
| 出版者 | ||||||||
| 出版者 | Austin Publishers | |||||||
| 関係URI | ||||||||
| 識別子タイプ | HDL | |||||||
| 関連識別子 | http://hdl.handle.net/10069/00040613 | |||||||
| 学位名 | ||||||||
| 学位名 | 博士(医学) | |||||||
| 学位授与機関 | ||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||
| 学位授与機関識別子 | 17301 | |||||||
| 学位授与機関名 | Nagasaki University (長崎大学) | |||||||
| 学位授与年月日 | ||||||||
| 学位授与年月日 | 2021-03-22 | |||||||
| 学位授与番号 | ||||||||
| 学位授与番号 | 甲医歯薬第1344号 | |||||||
| 学位の種類 | ||||||||
| 値 | 課程博士 | |||||||
| 引用 | ||||||||
| 内容記述タイプ | Other | |||||||
| 内容記述 | Nagasaki University (長崎大学), 博士(医学) (2021-03-22) | |||||||
