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Influence of age in weeks on the development and progression of nonalcoholic steatohepatitis in a diet-induced Sprague-Dawley rat model
http://hdl.handle.net/10069/00040789
http://hdl.handle.net/10069/000407892e7ebe85-c925-48b3-8a87-cff1faebfdd6
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ActMed65_7.pdf (774.5 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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| 公開日 | 2021-06-16 | |||||
| タイトル | ||||||
| タイトル | Influence of age in weeks on the development and progression of nonalcoholic steatohepatitis in a diet-induced Sprague-Dawley rat model | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Nonalcoholic steatohepatitis | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Animal model | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | High-fat high-cholesterol diet | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Age | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| 著者 |
Omagari, Katsuhisa
× Omagari, Katsuhisa× Yamasaki, Mayu× Ngo, Linh Chi Thi× Koba, Chiaki× Nagata, Asuka× Fukuda, Ayumi× Suruga, Kazuhito× Ichimura-Shimizu, Mayuko× Tsuneyama, Koichi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background: Understanding the pathogenesis of nonalcoholic steatohepatitis (NASH) in humans has been hampered by the lack of a comprehensive and physiological small animal model of NASH. We previously reported a dietary (high-fat and highcholesterol; HFC diet) -induced NASH model that developed advanced fibrosis within a relatively short period (9 weeks) using Sprague-Dawley (SD) rats (age, 9 weeks). Methods: In this study, we evaluated the age-related alterations of NASH in 9-, 18-, and 27-week-old male SD rats that were fed an HFC diet (30% fat, 1.25% cholesterol, and 0.5% sodium cholate, w/w) for 9 weeks (six rats/group). Results: Age-dependent increases in serum transaminases, insulin, and insulin resistance index were observed with or without a significant difference after the 9-week rearing period. Histopathological findings such as hepatic steatosis, lobular inflammation, and hepatocyte ballooning were similar regardless of age, but hepatic fibrosis was more evident in the older groups. Rats in all three groups developed NASH at a high rate (83.3% or higher in each group). The mRNA levels of fibrosisrelated genes encoding transforming growth factor-β (TGF-β) and α-smooth muscle actin (α-SMA) in the liver were low in the youngest group and high in the older groups, although this difference was not statistically significant. Conclusion: These results and those from our previous study indicate that a 9-week HFC diet-induced NASH model using SD rats can be applied a relatively wide range of ages (5-27 weeks of old), and that the risk of NASH-related fibrosis increases with age. |
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| 書誌情報 |
Acta medica Nagasakiensia 巻 65, 号 1, p. 7-15, 発行日 2021-06 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00016055 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00508430 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 出版者 | ||||||
| 出版者 | Nagasaki University School of Medicine | |||||
| 出版者別言語 | ||||||
| 値 | 長崎大学医学部 | |||||
| sortkey | ||||||
| 値 | 02 | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Acta medica Nagasakiensia, 65(1), pp.7-15; 2021 | |||||
