WEKO3
アイテム
Rab3a, a small GTP-binding protein, is required for the stabilization of the murine leukaemia virus Gag protein
http://hdl.handle.net/10069/00041933
http://hdl.handle.net/10069/00041933366b050a-df56-4d69-b949-0e659ca3227c
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
SGTP13_162.pdf (18.1 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2022-12-13 | |||||
| タイトル | ||||||
| タイトル | Rab3a, a small GTP-binding protein, is required for the stabilization of the murine leukaemia virus Gag protein | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Murine leukaemia virus | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | gag protein | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Rab3a | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | CD63 | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Izumida, Mai
× Izumida, Mai× Kakoki, Katsura× Hayashi, Hideki× Matsuyama, Toshifumi× Kubo, Yoshinao |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We recently identified a CD63-interacting protein to understand the role of CD63 in virion production of the human immunodeficiency virus type 1, and we have found that Rab3a forms a complex with CD63. In this study, we analysed the effect of Rab3a on virion production of the murine leukaemia virus (MLV), which is another member of the retrovirus family. We found that Rab3a silencing induced lysosomal degradation of the MLV Gag protein, and recovery of the Rab3a expression restored the level of the Gag protein through a complex formation of MLV Gag and Rab3a, indicating that Rab3a is required for MLV Gag protein expression. In contrast, CD63 silencing decreased the infectivity of released virions but had no effect on virion production, indicating that CD63 facilitates the infectivity of released MLV particles. Although Rab3a induced CD63 degradation in uninfected cells, the complex of MLV Gag and Rab3a suppressed the Rab3a-mediated CD63 degradation in MLV-infected cells. Finally, we found that the MLV Gag protein interacts with Rab3a to stabilize its own protein and CD63 that facilitates the infectivity of released MLV particles. Considering the involvement of Rab3a in lysosome trafficking to the plasma membrane, it may also induce cell surface transport of the MLV Gag protein. | |||||
| 書誌情報 |
Small GTPases 巻 13, 号 1, p. 162-182, 発行日 2021-06-27 |
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| 出版者 | ||||||
| 出版者 | Taylor and Francis Ltd | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 2154-1248 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1080/21541248.2021.1939631 | |||||
| 権利 | ||||||
| 権利情報 | This is an Accepted Manuscript of an article published by Taylor & Francis in Small GTPases on 27 Jun 2021, available online: http://www.tandfonline.com/10.1080/21541248.2021.1939631. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Small GTPases, 13(1), pp.162-182; 2022 | |||||
