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An Amphipathic Structure of a Dipropylglycine-Containing Helical Peptide with Sufficient Length Enables Safe and Effective Intracellular siRNA Delivery
http://hdl.handle.net/10069/00042138
http://hdl.handle.net/10069/00042138fa09ce0e-73c1-4da7-b094-998f5c219928
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
CPB71_250.pdf (3.2 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2023-03-24 | |||||
| タイトル | ||||||
| タイトル | An Amphipathic Structure of a Dipropylglycine-Containing Helical Peptide with Sufficient Length Enables Safe and Effective Intracellular siRNA Delivery | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | cell-penetrating peptide | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | small interfering RNA (siRNA) delivery | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | helical structure | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | amphipathic structure | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | unnatural amino acid | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Oba, Makoto
× Oba, Makoto× Shibuya, Mika× Yamaberi, Yuto× Yokoo, Hidetomo× Uchida, Satoshi× Ueda, Atsushi× Tanaka, Masakazu |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Amphipathic peptides composed of cationic amino acids and hydrophobic amino acids have cell-penetrating ability and are often used as a delivery tool for membrane-impermeable compounds. Small interfering RNA (siRNAs) are one of the delivery targets for such cell-penetrating peptides (CPPs). Cationic CPPs can associate with anionic siRNAs by electrostatic interactions resulting in the formation of nano-sized complexes, which can deliver siRNAs intracellularly. CPPs containing unnatural amino acids offer promising tools to siRNA delivery. However, the detailed structure–activity relationship in siRNA delivery has been rarely studied. In the current study, we designed peptides containing dipropylglycine (Dpg) and explored the cellular uptake and cytotoxicity of peptide/siRNA complexes. The amphipathic structure of the peptides played a key role in complexation with siRNAs and intracellular siRNA delivery. In the amphipathic peptides, cellular uptake of siRNA increased with increasing peptide length, but cytotoxicity was reduced. A peptide containing four Dpg exhibited an effective gene-silencing effect with small amounts of peptides without cytotoxicity in medium containing serum. These findings will be helpful for the design of novel CPPs for siRNA delivery. | |||||
| 書誌情報 |
Chemical and Pharmaceutical Bulletin 巻 71, 号 3, p. 250-256, 発行日 2023-03-01 |
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| 出版者 | ||||||
| 出版者 | Pharmaceutical Society of Japan | |||||
| 出版者別言語 | ||||||
| 値 | 日本薬学会 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0009-2363 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1248/cpb.c22-00852 | |||||
| 権利 | ||||||
| 権利情報 | © 2023 The Pharmaceutical Society of Japan | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Chemical & pharmaceutical bulletin, 71(3), pp. 250-256; 2023 | |||||
