WEKO3
アイテム
Enterokinase Enhances Influenza A Virus Infection by Activating Trypsinogen in Human Cell Lines
http://hdl.handle.net/10069/38239
http://hdl.handle.net/10069/38239ca54aba0-bf7f-4877-9a09-e7f9dd879dd3
名前 / ファイル | ライセンス | アクション |
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FCIM8_91.pdf (2.5 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-05-31 | |||||
タイトル | ||||||
タイトル | Enterokinase Enhances Influenza A Virus Infection by Activating Trypsinogen in Human Cell Lines | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Enterokinase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Genome structure and function | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Hemagglutinin processing | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Influenza A virus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Transmembrane serine protease | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Hayashi, Hideki
× Hayashi, Hideki× Kubo, Yoshinao× Izumida, Mai× Takahashi, Etsuhisa× Kido, Hiroshi× Sato, Ko× Yamaya, Mutsuo× Nishimura, Hidekazu× Nakayama, Kou× Matsuyama, Toshifumi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Cleavage and activation of hemagglutinin (HA) by trypsin-like proteases in influenza A virus (IAV) are essential prerequisites for its successful infection and spread. In host cells, some transmembrane serine proteases such as TMPRSS2, TMPRSS4 and HAT, along with plasmin in the bloodstream, have been reported to cleave the HA precursor (HA0) molecule into its active forms, HA1 and HA2. Some trypsinogens can also enhance IAV proliferation in some cell types (e.g., rat cardiomyoblasts). However, the precise activation mechanism for this process is unclear, because the expression level of the physiological activator of the trypsinogens, the TMPRSS15 enterokinase, is expected to be very low in such cells, with the exception of duodenal cells. Here, we show that at least two variant enterokinases are expressed in various human cell lines, including A549 lung-derived cells. The exogenous expression of these enterokinases was able to enhance the proliferation of IAV in 293T human kidney cells, but the proliferation was reduced by knocking down the endogenous enterokinase in A549 cells. The enterokinase was able to enhance HA processing in the cells, which activated trypsinogen in vitro and in the IAV-infected cells also. Therefore, we conclude that enterokinase plays a role in IAV infection and proliferation by activating trypsinogen to process viral HA in human cell lines. | |||||
書誌情報 |
Frontiers in Cellular and Infection Microbiology 巻 8, p. 91, 発行日 2018-03-23 |
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出版者 | ||||||
出版者 | Frontiers Media S.A. | |||||
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収録物識別子タイプ | ISSN | |||||
収録物識別子 | 22352988 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.3389/fcimb.2018.00091 | |||||
権利 | ||||||
権利情報 | c 2018 Hayashi, Kubo, Izumida, Takahashi, Kido, Sato, Yamaya, Nishimura, Nakayama and Matsuyama. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Frontiers in Cellular and Infection Microbiology, 8, 91; 2018 |