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Splenic Delivery System of pDNA through Complexes Electrostatically Constructed with Protamine and Chondroitin Sulfate
http://hdl.handle.net/10069/38244
http://hdl.handle.net/10069/38244fa605df7-62ca-4268-b82d-b139d3c1c477
名前 / ファイル | ライセンス | アクション |
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BPBul41_342.pdf (2.0 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2018-05-31 | |||||
タイトル | ||||||
タイトル | Splenic Delivery System of pDNA through Complexes Electrostatically Constructed with Protamine and Chondroitin Sulfate | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Chondroitin sulfate sodium | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Gene delivery | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Medical product | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Plasmid DNA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Protamine sulfate | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kodama, Yukinobu
× Kodama, Yukinobu× Nishigaki, Waka× Nakamura, Tadahiro× Fumoto, Shintaro× Nishida, Koyo× Kurosaki, Tomoaki× Nakagawa, Hiroo× Kitahara, Takashi× Muro, Takahiro× Sasaki, Hitoshi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We developed and optimized a novel gene delivery vector constructed electrostatically with an anionic biological component and a cationic biological component. Cationic binary complexes of plasmid DNA (pDNA) with novo-protamine sulfate as a medical product (PRT complexes) demonstrated high gene expression with minimal cytotoxicity, likely related with its total cationic charge. Subsequently, anionic compounds were added to the PRT complexes to form ternary complexes with neutral or anionic charges. Among the anionic compounds examined, chondroitin sulfate sodium (CS) as a medical product encapsulated the PRT complexes to produce stable ternary complexes (CS complexes) at charge ratios of ?4 with pDNA. CS complexes exhibited high gene expression without cytotoxicity in mouse melanoma cell line, B16-F10 cells, in vitro. An inhibition study with endocytosis inhibitors suggested that PRT complexes were mainly taken up by caveolae-mediated endocytosis, and CS complexes were mainly taken up by clathrin-mediated endocytosis in B16-F10 cells. We found that CS complexes including pDNA encoding Oplophorus gracilirostris luciferase induced selective gene expression in the spleen after intravenous administration into ddY male mice. Thus, we successfully constructed useful gene vectors with biological components as medical products. | |||||
書誌情報 |
Biological and Pharmaceutical Bulletin 巻 41, 号 3, p. 342-349, 発行日 2018-03-01 |
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出版者 | ||||||
出版者 | 日本薬学会 | |||||
出版者別言語 | ||||||
Pharmaceutical Society of Japan | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09186158 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13475215 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1248/bpb.b17-00667 | |||||
権利 | ||||||
権利情報 | c 2018 The Pharmaceutical Society of Japan. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biological and Pharmaceutical Bulletin, 41(3), pp.342-349; 2018 |