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TNF-α inhibits the growth of Legionella pneumophila in airway epithelial cells by inducing apoptosis
http://hdl.handle.net/10069/37166
http://hdl.handle.net/10069/37166687a7fd9-7eb9-4d3a-93f7-9f43035ebb12
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ISYK927_Kawamoto.pdf (585.6 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2017-04-18 | |||||
| タイトル | ||||||
| タイトル | TNF-α inhibits the growth of Legionella pneumophila in airway epithelial cells by inducing apoptosis | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Anti-TNF-α therapy | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Cell death | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Caspase 3 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Caspase 7 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Lung epithelial cells | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 著者 |
川元, 康嗣
× 川元, 康嗣 |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 115603 | |||||
| 姓名 | Kawamoto, Yasuhide | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Background: TNF-α plays an important role in the pathogenesis of Legionella pneumophila (Lp)-induced pneumonia. Patients undergoing anti-TNF-α therapy are at an increased risk of Lp infection. Lp infects both phagocytic and non-phagocytic cells such as airway epithelial cells; however, the role of TNF-α in airway epithelial cells is unknown. Methods: Human airway epithelial cell line NCI-H292 was infected with Lp NUL1 strain. After infection, both intracellular growth of Lp and cell death were evaluated after treating the cells with or without TNF-α. Apoptosis was examined by performing activated caspase-3/7 staining and by using a pan-caspase inhibitor. Results: Lp infected and replicated in NCI-H292 cells in a time-dependent manner, and TNF-α treatment of Lp-infected NCI-H292 cells inhibited Lp replication. Inhibitory effects of TNF-α on Lp replication were suppressed after treatment with a TNF-α-neutralizing antibody. Lp infection increased extracellular lactate dehydrogenase levels and decreased the number of living cells. Increased number of Lp-infected NCI-H292 cells showed caspase-3/7 activation, indicating they underwent apoptosis. TNF-α treatment inhibited Lp replication by increasing the apoptosis of NCI-H292 cells. Conclusions: Thus, our results suggested that airway epithelial cells were involved in the pathogenesis of Lp infection and that TNF-α played a protective role by inhibiting the intracellular replication of Lp and by increasing the apoptosis of Lp-infected airway epithelial cells. However, Lp infection should be investigated further in patients undergoing anti-TNF-α therapy who develop pneumonia. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 長崎大学学位論文 学位記番号:博(医歯薬)甲第927号 学位授与年月日:平成29年2月22日 | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Author: Yasuhide Kawamoto, Yoshitomo Morinaga, Yumiko Kimura, Norihito Kaku, Kosuke Kosai, Naoki Uno, Hiroo Hasegawa, Katsunori Yanagihara | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Citation: Journal of Infection and Chemotherapy, 23(1), pp.51?55; 2017 | |||||
| 書誌情報 |
Journal of Infection and Chemotherapy 巻 23, 号 1, p. 51-55, 発行日 2017-02-22 |
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| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1341321X | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.jiac.2016.09.010 | |||||
| 権利 | ||||||
| 権利情報 | c 2016 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved. |
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| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| その他のタイトル | ||||||
| その他のタイトル | TNF-αは、アポトーシスを誘導することでLegionella pneumophilaの気道上皮細胞内での増殖を抑制する | |||||
| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| 学位名 | ||||||
| 学位名 | 博士(医学) | |||||
| 学位授与機関 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 17301 | |||||
| 学位授与機関名 | Nagasaki University (長崎大学) | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2017-02-22 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 甲医歯薬第927号 | |||||
| 学位の種類 | ||||||
| 値 | 課程博士 | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Nagasaki University (長崎大学), 博士(医学) (2017-02-22) | |||||
