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Evaluation of the tetrodotoxin uptake ability of pufferfish Takifugu rubripes tissues according to age using an in vitro tissue slice incubation method
http://hdl.handle.net/10069/39579
http://hdl.handle.net/10069/395798f13a06e-e37e-45e0-9f77-f6cac2273d52
名前 / ファイル | ライセンス | アクション |
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Toxicon174_8.pdf (648.8 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-11-27 | |||||
タイトル | ||||||
タイトル | Evaluation of the tetrodotoxin uptake ability of pufferfish Takifugu rubripes tissues according to age using an in vitro tissue slice incubation method | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Immunohistochemistry | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Pufferfish | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Takifugu rubripes | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Tetrodotoxin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Tissue slice | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Gao, Wei
× Gao, Wei× Yamada, Misako× Ohki, Rieko× Nagashima, Yuji× Tatsuno, Ryohei× Ikeda, Koichi× Kawatsu, Kentaro× Takatani, Tomohiro× Arakawa, Osamu |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The tetrodotoxin (TTX) uptake ability of pufferfish Takifugu rubripes tissues and its growth-associated changes were investigated using an in vitro tissue slice incubation method. Tissue slices prepared from the liver, skin, and intestine of a non-toxic cultured adult T. rubripes (20 months old) and incubated with incubation buffer containing 25 μg/mL TTX for 1?48 h showed a time-dependent increase in the TTX content in all tissues. The TTX contents of the skin and intestine slices were comparable to or slightly higher than that of the liver slices, with a similar transition pattern, suggesting similar TTX uptake ability among the skin, intestine, and liver. The TTX uptake ability of the liver and intestine did not differ significantly between young (8 months old) and adult (20 months old) fish, but the skin slices of young fish took up approximately twice as much TTX as that of adult fish, suggesting that the TTX uptake ability of the skin is involved in the growth-dependent changes in the toxin distribution inside the body in T. rubripes. To estimate the TTX uptake pathway in each tissue, an immunohistochemical technique was used to observe temporal changes in the intra-tissue microdistribution of TTX during incubation. The findings suggested that TTX is transferred and accumulates from pancreatic exocrine cells to hepatic parenchymal cells in the liver, from connective tissues to basal cells in the skin, and from villi epithelial cells via the lamina propria to the muscle layer in the intestine. | |||||
書誌情報 |
Toxicon 巻 174, p. 8-12, 発行日 2019-11-27 |
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出版者 | ||||||
出版者 | Elsevier Ltd. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00410101 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.toxicon.2019.11.014 | |||||
権利 | ||||||
権利情報 | c 2019 Elsevier Ltd. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Toxicon, 174, pp.8-12; 2019 |