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アイテム
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Quaternary Complexes Modified from pDNA and Poly-l-Lysine Complexes to Enhance pH-Buffering Effect and Suppress Cytotoxicity
http://hdl.handle.net/10069/35263
http://hdl.handle.net/10069/35263e25dfd3a-b74d-427e-86e2-5416bb5375b3
名前 / ファイル | ライセンス | アクション |
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JPS104_1470.pdf (2.4 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2016-05-01 | |||||
タイトル | ||||||
タイトル | Quaternary Complexes Modified from pDNA and Poly-l-Lysine Complexes to Enhance pH-Buffering Effect and Suppress Cytotoxicity | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | biocompatibility | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | biodegradable polymers | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | nanoparticles | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | non-viral gene delivery | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | plasmid DNA | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kodama, Yukinobu
× Kodama, Yukinobu× Yatsugi, Yuiko× Kitahara, Takashi× Kurosaki, Tomoaki× Egashira, Kanoko× Nakashima, Mikiro× Muro, Takahiro× Nakagawa, Hiroo× Higuchi, Norihide× Nakamura, Tadahiro× Sasaki, Hitoshi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We developed a modified complex of pDNA and poly-l-lysine (PLL) by the addition of poly-l-histidine (PLH) and γ-polyglutamic acid (γ-PGA) to enhance its pH-buffering effect and suppress cytotoxicity. The binary and ternary complexes of pDNA with PLL or/and PLH showed particle sizes of approximately 52-76 nm with cationic surface charge. The ternary complexes showed much higher gene expression than the binary complexes with PLL. The mixed solution of PLL and PLH showed higher buffering capacity than PLL solution. The high gene expression of ternary complexes was reduced by bafilomycin A1. These results indicated the addition of PLH to PLL complexes promoted endosomal escape by enhancing the pH-buffering effect. The binary and ternary complexes showed cytotoxicity and blood agglutination because of their cationic surface charge. We therefore developed quaternary complexes by the addition of anionic γ-PGA, which was reported to decrease the toxicity of cationic complexes. In fact, quaternary complexes showed no cytotoxicity and blood agglutination. Also, quaternary complexes showed higher gene expression than ternary complexes regardless of their anionic surface charge. Quaternary complexes showed selectively high gene expression in the spleen after their intravenous administration. Thus, we successfully developed the quaternary complexes with high gene expression and no toxicity. | |||||
書誌情報 |
Journal of Pharmaceutical Sciences 巻 104, 号 4, p. 1470-1477, 発行日 2015-04 |
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出版者 | ||||||
出版者 | John Wiley and Sons Inc. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00223549 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1002/jps.24364 | |||||
権利 | ||||||
権利情報 | c 2015 Wiley Periodicals, Inc. and the American Pharmacists Association | |||||
権利 | ||||||
権利情報 | This is the accepted version of the following article: Journal of Pharmaceutical Sciences, 104(4), pp.1470-1477; 2015, which has been published in final form at http://dx.doi.org/10.1002/jps.24364 | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of Pharmaceutical Sciences, 104(4), pp.1470-1477; 2015 |