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Carbon Nanofiber-based Luminol-biotin Probe for Sensitive Chemiluminescence Detection of Protein
http://hdl.handle.net/10069/35280
http://hdl.handle.net/10069/3528033b8740e-f5df-4cf9-bdf4-662c7858bd68
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
AnaSci30_1051.pdf (748.6 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-05-08 | |||||
| タイトル | ||||||
| タイトル | Carbon Nanofiber-based Luminol-biotin Probe for Sensitive Chemiluminescence Detection of Protein | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Carbon nanofiber | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Chemiluminescence | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Luminol | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Proteins | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Streptavidin | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Baj, Stefan
× Baj, Stefan× Krawczyk, Tomasz× Pradel, Natalia× Azam, Md. Golam× Shibata, Takayuki× Dragusha, Shpend× Skutil, Krzysztof× Pawlyta, Miroslawa× Kai, Masaaki |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | A carbon nanofiber-based luminol-biotin probe was synthesized for the sensitive chemiluminescence (CL) detection of a target protein by grafting luminol and biotin onto an oxidized carbon nanofiber. This carbon nanofiber was prepared by chemical vapor-deposition with methane in the presence of the Ni-Cu-MgO catalyst, which was followed by oxidization with HNO3-H2SO4 to produce a carboxyl group on the surface of the nanofiber. The material was grafted with luminol and biotin by means of a standard carbodiimide activation of COOH groups to produce corresponding amides. The substance was water-soluble and thus could be utilized as a sensitive CL probe for a protein assay. The probe showed highly specific affinity towards the biotin-labeled antibody via a streptavidin-biotin interaction. The detection limit for this model assay was approximately 0.2 pmol of the biotinized IgG spotted on a polyvinylidene fluoride (PVDF) membrane. Nonspecific binding to other proteins was not observed. Therefore, the synthesized carbon nanofiber-based CL probe may be useful for a sensitive and specific analysis of the target protein. | |||||
| 書誌情報 |
Analytical Sciences 巻 30, 号 11, p. 1051-1056, 発行日 2014-11-10 |
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| 出版者 | ||||||
| 出版者 | 日本分析化学会 | |||||
| 出版者別言語 | ||||||
| 値 | Japan Society for Analytical Chemistry | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 09106340 | |||||
| EISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 13482246 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.2116/analsci.30.1051 | |||||
| 権利 | ||||||
| 権利情報 | c 2014 by The Japan Society for Analytical Chemistry | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Analytical Sciences, 30(11), pp.1051-1056; 2014 | |||||
