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Fluorometric assay for phenotypic differentiation of drug-resistant HIV mutants
http://hdl.handle.net/10069/35502
http://hdl.handle.net/10069/35502ad6c2526-3859-4394-bb6a-e59c3777863e
名前 / ファイル | ライセンス | アクション |
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SciRep5_10323.pdf (1.2 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2015-06-29 | |||||
タイトル | ||||||
タイトル | Fluorometric assay for phenotypic differentiation of drug-resistant HIV mutants | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Zhu, Qinchang
× Zhu, Qinchang× Yu, Zhiqiang× Kabashima, Tsutomu× Yin, Sheng× Dragusha, Shpend× El-Mahdy, Ahmed F. M.× Ejupi, Valon× Shibata, Takayuki× Kai, Masaaki |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Convenient drug-resistance testing of viral mutants is indispensable to effective treatment of viral infection. We developed a novel fluorometric assay for phenotypic differentiation of drug-resistant mutants of human immunodeficiency virus-I protease (HIV-PR) which uses enzymatic and peptide-specific fluorescence (FL) reactions and high-performance liquid chromatography (HPLC) of three HIV-PR substrates. This assay protocol enables use of non-purified enzyme sources and multiple substrates for the enzymatic reaction. In this study, susceptibility of HIV mutations to drugs was evaluated by selective formation of three FL products after the enzymatic HIV-PR reaction. This proof-of-concept study indicates that the present HPLC-FL method could be an alternative to current phenotypic assays for the evaluation of HIV drug resistance. | |||||
書誌情報 |
Scientific Reports 巻 5, p. 10323, 発行日 2015-05-19 |
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出版者 | ||||||
出版者 | Nature Publishing Group | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 20452322 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1038/srep10323 | |||||
権利 | ||||||
権利情報 | This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Scientific Reports, 5, 10323; 2015 |