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Endocytosis of Multiwalled Carbon Nanotubes in Bronchial Epithelial and Mesothelial Cells
http://hdl.handle.net/10069/35621
http://hdl.handle.net/10069/3562127272075-5c63-43dd-84f2-30a9602f749f
名前 / ファイル | ライセンス | アクション |
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BMRI2015_793186.pdf (2.1 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2015-07-09 | |||||
タイトル | ||||||
タイトル | Endocytosis of Multiwalled Carbon Nanotubes in Bronchial Epithelial and Mesothelial Cells | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Maruyama, Kayo
× Maruyama, Kayo× Haniu, Hisao× Saito, Naoto× Matsuda, Yoshikazu× Tsukahara, Tamotsu× Kobayashi, Shinsuke× Tanaka, Manabu× Aoki, Kaoru× Takanashi, Seiji× Okamoto, Masanori× Kato, Hiroyuki |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Bronchial epithelial cells and mesothelial cells are crucial targets for the safety assessment of inhalation of carbon nanotubes (CNTs), which resemble asbestos particles in shape. Intrinsic properties of multiwalled CNTs (MWCNTs) are known to cause potentially hazardous effects on intracellular and extracellular pathways. These interactions alter cellular signaling and affect major cell functions, resulting in cell death, lysosome injury, reactive oxygen species production, apoptosis, and cytokine release. Furthermore, CNTs are emerging as a novel class of autophagy inducers. Thus, in this study, we focused on the mechanisms of MWCNT uptake into the human bronchial epithelial cells (HBECs) and human mesothelial cells (HMCs). We verified that MWCNTs are actively internalized into HBECs and HMCs and were accumulated in the lysosomes of the cells after 24-hour treatment. Next, we determined which endocytosis pathways (clathrin-mediated, caveolae-mediated, and macropinocytosis) were associated with MWCNT internalization by using corresponding endocytosis inhibitors, in two nonphagocytic cell lines derived from bronchial epithelial cells and mesothelioma cells. Clathrin-mediated endocytosis inhibitors significantly suppressed MWCNT uptake, whereas caveolae-mediated endocytosis and macropinocytosis were also found to be involved in MWCNT uptake. Thus, MWCNTs were positively taken up by nonphagocytic cells, and their cytotoxicity was closely related to these three endocytosis pathways. | |||||
書誌情報 |
BioMed Research International 巻 2015, p. 1-9, 発行日 2015 |
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出版者 | ||||||
出版者 | Hindawi Publishing Corporation | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 23146133 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 23146141 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1155/2015/793186 | |||||
権利 | ||||||
権利情報 | c 2015 Kayo Maruyama et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | BioMed Research International, 2015, 793186; 2015 |