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ABCC11/MRP8 Expression in the Gastrointestinal Tract and a Novel Role for Pepsinogen Secretion
http://hdl.handle.net/10069/35903
http://hdl.handle.net/10069/3590388d4650b-ee30-4322-b971-a07f79d80f17
名前 / ファイル | ライセンス | アクション |
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ISYK722_Matsumoto.pdf (3.2 MB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2015-10-22 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | ABCC11/MRP8 Expression in the Gastrointestinal Tract and a Novel Role for Pepsinogen Secretion | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ABC transporter | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ABCC11/MRP8 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Gastric chief cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Gastrointestinal tract | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Pepsinogen I | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
著者 |
松本, 博文
× 松本, 博文 |
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著者別名 | ||||||
姓名 | Matsumoto, Hirofumi | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | ATP-binding cassette (ABC) transporters are involved in chemotherapy resistance. Multidrug-resistance protein 8 (ABCC11/MRP8) is also involved in 5-fluorouracil (5-FU) metabolism. 5-FU and its derivatives are widely used in the treatment of gastrointestinal tract cancers, but little is known about the contribution of ABCC11/MRP8 to gastrointestinal tract and related cancers. Here, we report our investigation of ABCC11/MRP8 expression in normal and cancerous gastrointestinal tract tissues and reveal its novel role in the gastric mucosa. In tissue microarray and surgically resected cancer specimens, immunohistochemical (IHC) staining revealed significantly reduced expression of ABCC11/ MRP8 in gastrointestinal tract cancers compared with other cancers. In contrast, strong ABCC11/MRP8 expression was observed in normal gastric mucosa. Additional immuno-fluorescence assays revealed co-localization of ABCC11/MRP8 and pepsinogen I in normal gastric chief cells. Quantitative PCR and Western blot analysis also revealed significant expression of ABCC11/MRP8 in fundic mucosa where the chief cells are mainly located. Furthermore, the ABCC11 mRNA-suppressed NCI-N87 gastric cancer cell line failed to secret pepsinogen I extracellularly. Thus, low expression of ABCC11/MRP8 is consistent with chemotherapeutic regimens using 5-FU and its derivatives in gastrointestinal tract cancers. Our results indicated a novel function of ABCC11/MRP8 in the regulation of pepsinogen I secretion in the normal gastric chief cells. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 長崎大学学位論文 学位記番号:博(医歯薬)甲第722号 学位授与年月日:平成26年10月1日 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Author: Hirofumi Matsumoto, Tomoshi Tsuchiya, Koh-ichiro Yoshiura, Tomayoshi Hayashi, Shigekazu Hidaka, Atsushi Nanashima, Takeshi Nagayasu | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Citation: ACTA HISTOCHEMICA ET CYTOCHEMICA, 47(3), pp.85-94; 2014 | |||||
書誌情報 |
ACTA HISTOCHEMICA ET CYTOCHEMICA 巻 47, 号 3, p. 85-94, 発行日 2014-10-01 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00445991 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13475800 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1267/ahc.13040 | |||||
権利 | ||||||
権利情報 | c 2014 The Japan Society of Histochemistry and Cytochemistry. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
その他のタイトル | ||||||
その他のタイトル | ABCC11/MRP8の消化管における発現とペプシノーゲン産生における新規的役割 | |||||
出版者 | ||||||
出版者 | 日本組織細胞化学会 | |||||
関係URI | ||||||
識別子タイプ | HDL | |||||
関連識別子 | http://hdl.handle.net/10069/35358 | |||||
学位名 | ||||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 17301 | |||||
学位授与機関名 | Nagasaki University (長崎大学) | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2014-10-01 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲医歯薬第722号 | |||||
学位の種類 | ||||||
課程博士 | ||||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Nagasaki University (長崎大学), 博士(医学) (2014-10-01) |