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RELEVANCE OF MOLECULAR TESTS FOR HTLV-1 INFECTION AS CONFIRMATORY TESTS AFTER THE FIRST SERO-SCREENING
http://hdl.handle.net/10069/34177
http://hdl.handle.net/10069/34177c934d893-464d-4039-a020-0b5f29bc0168
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
JII35_74.pdf (489.0 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2015-01-03 | |||||
| タイトル | ||||||
| タイトル | RELEVANCE OF MOLECULAR TESTS FOR HTLV-1 INFECTION AS CONFIRMATORY TESTS AFTER THE FIRST SERO-SCREENING | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | CLEIA | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | CLIA | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | HTLV-1 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | PCR | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | westernblot | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Ishihara, Kaori
× Ishihara, Kaori× Inokuchi, Naoko× Tsushima, Yuko× Tsuruda, Kazuto× Morinaga, Yoshitomo× Hasegawa, Hiroo× Yanagihara, Katsunori× Kamihira, Shimeru |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The diagnosis of human T-cell leukemia virus type-1 (HTLV-1) infection has been widely examined by serologics. In the first screening tests, serological false negative and positive samples have been reduced thanks to advances in assay techniques that apply new emission agents and sensors. On the other hand, western blot (WB) remains problematic. For example, WB analysis yields many samples equivalent to antibody positive ones. To reduce the need for WB, an alternative testing strategy is required to detect HTLV-1 infection. Polymerase chain reaction (PCR) for the HTLV-1 provirus has recently been recommended for a final diagnosis of infection. However, although PCR is thought to be one element, the validation of detection performance for HTLV-1 infection between serological and molecular testing is not always clear. Thus, this study aimed to evaluate the accuracy and test the validity of an improved methodology for serological detection of HTLV-infection, as well as that of PCR. In conclusion, the high values of kappa-statistics are expected to deliver high quality in chemiluminescent enzyme immunoassay (or chemiluminescent immunoassay), while the problems with WB assays remain to be elucidated. As an alternative to WB, a combination of real-time qPCR and nested PCR is proposed as a suitable confirmatory test. | |||||
| 書誌情報 |
Journal of Immunoassay and Immunochemistry 巻 35, 号 1, p. 74-82, 発行日 2014-01-02 |
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| 出版者 | ||||||
| 出版者 | Taylor and Francis Inc. | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 15321819 | |||||
| EISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 15324230 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1080/15321819.2013.792832 | |||||
| 権利 | ||||||
| 権利情報 | This is an Author's Accepted Manuscript of an article published in Journal of Immunoassay and Immunochemistry, 35(1), pp.74-82; 2014 c Taylor & Francis, available online at: http://www.tandfonline.com/10.1080/15321819.2013.79283 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Journal of Immunoassay and Immunochemistry, 35(1), pp.74-82; 2014 | |||||
