WEKO3
アイテム
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Development of anionic bubble lipopolyplexes for efficient and safe gene transfection with ultrasound exposure in mice
http://hdl.handle.net/10069/34194
http://hdl.handle.net/10069/3419419bfbc6e-4c01-4831-bb9b-ea5c9a1457a1
名前 / ファイル | ライセンス | アクション |
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JCR176_24.pdf (1.2 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-03-13 | |||||
タイトル | ||||||
タイトル | Development of anionic bubble lipopolyplexes for efficient and safe gene transfection with ultrasound exposure in mice | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Biocompatibility | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Liposomes | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Self assembly | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Sonoporation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Transfection | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kurosaki, Tomoaki
× Kurosaki, Tomoaki× Kawakami, Shigeru× Higuchi, Yuriko× Suzuki, Ryo× Maruyama, Kazuo× Sasaki, Hitoshi× Yamashita, Fumiyoshi× Hashida, Mitsuru |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Anionic bubble lipopolyplexes have been developed as anionic ultrasound (US)-responsive gene delivery carriers with biocompatible compounds for efficient and safe transfection in mice. The particles of the anionic bubble lipopolyplexes were approximately 450-600 nm with an anionic surface charge. In the absence of US exposure, the bubble lipopolyplexes showed extremely low gene expression in the human vascular endothelial cell line EAhy926. The anionic bubble lipopolyplexes, however, delivered pDNA into cells without endocytosis and showed markedly high gene expression following US exposure. The anionic bubble lipopolyplexes showed little cytotoxicity in EAhy926 cells and little aggregation with erythrocytes. Following intravenous administration into mice, the anionic bubble lipopolyplexes showed high levels of gene expression in the liver, kidney, and spleen only after US exposure to the abdominal area. The level of gene expression in liver non-parenchymal cells was significantly higher than that in parenchymal cells. In addition, the anionic bubble lipopolyplexes did not show any severe hepatic toxicity and did not enhance the production of proinflammatory cytokines. Overall, we have succeeded in preparing anionic bubble lipopolyplexes for efficient and safe transfection with US exposure in mice. | |||||
書誌情報 |
Journal of Controlled Release 巻 176, p. 24-34, 発行日 2014-02-28 |
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出版者 | ||||||
出版者 | Elsevier | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 01683659 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.jconrel.2013.12.023 | |||||
権利 | ||||||
権利情報 | c 2013 Elsevier B.V. | |||||
権利 | ||||||
権利情報 | NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Controlled Release. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Controlled Release, 176, 1, (2014) | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of Controlled Release, 176, pp.24-34; 2014 |