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Influence of pH on Extracellular Matrix Preservation During Lung Decellularization
http://hdl.handle.net/10069/34991
http://hdl.handle.net/10069/34991b5f7ff1d-f3ba-4c16-ae5d-0ccc8b27e011
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
TE-CMet20_1028.pdf (5.2 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2015-12-02 | |||||
| タイトル | ||||||
| タイトル | Influence of pH on Extracellular Matrix Preservation During Lung Decellularization | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Decellularization | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Lung | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Tissue engineering | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | ECM (extracellular matrix) | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | DNA | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Tsuchiya, Tomoshi
× Tsuchiya, Tomoshi× Balestrini, Jenna L.× Mendez, Julio× Calle, Elizabeth A.× Zhao, Liping× Niklason, Laura E. |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The creation of decellularized organs for use in regenerative medicine requires the preservation of the organ extracellular matrix (ECM) as a means to provide critical cues for differentiation and migration of cells that are seeded onto the organ scaffold. The purpose of this study was to assess the influence of varying pH levels on the preservation of key ECM components during the decellularization of rat lungs. Herein, we show that the pH of the 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS)-based decellularization solution influences ECM retention, cell removal, and also the potential for host response upon implantation of acellular lung tissue. The preservation of ECM components, including elastin, fibronectin, and laminin, were better retained in the lower pH conditions that were tested (pH ranges tested: 8, 10, 12); glycosaminoglycans were preserved to a higher extent in the lower pH groups as well. The DNA content following decellularization of the rat lung was inversely correlated with the pH of the decellularization solution. Despite detectible levels of cyotoskeletal proteins and significant residual DNA, tissues decellularized at pH 8 demonstrated the greatest tissue architecture maintenance and the least induction of host response of all acellular conditions. These results highlight the effect of pH on the results obtained by organ decellularization and suggest that altering the pH of the solutions used for decellularization may influence the ability of cells to properly differentiate and home to appropriate locations within the scaffold, based on the preservation of key ECM components and implantation results. | |||||
| 書誌情報 |
Tissue Engineering Part C: Methods 巻 20, 号 12, p. 1028-1036, 発行日 2014-12-01 |
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| 出版者 | ||||||
| 出版者 | Mary Ann Liebert Inc. | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 19373384 | |||||
| EISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 19373392 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1089/ten.tec.2013.0492 | |||||
| 権利 | ||||||
| 権利情報 | c2014 Mary Ann Liebert, Inc. | |||||
| 権利 | ||||||
| 権利情報 | The final published version is available from Mary Ann Liebert, Inc., publishers at http://dx.doi.org/10.1089/ten.tec.2013.0492 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Tissue Engineering Part C: Methods, 20(12), pp.1028-1036; 2014 | |||||
