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Establishment of ATP-Based Luciferase Viability Assay in 96-Well Plate for Trypanosoma congolense
http://hdl.handle.net/10069/34999
http://hdl.handle.net/10069/34999d3971052-4975-4a25-ade3-ea159ac202e1
| 名前 / ファイル | ライセンス | アクション |
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JVMS76_1437.pdf (538.8 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||||
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| 公開日 | 2015-01-13 | |||||||||||||||||||
| タイトル | ||||||||||||||||||||
| タイトル | Establishment of ATP-Based Luciferase Viability Assay in 96-Well Plate for Trypanosoma congolense | |||||||||||||||||||
| 言語 | ||||||||||||||||||||
| 言語 | eng | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| 主題 | Animal African trypanosomosis | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| 主題 | Drug screening | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| 主題 | Luciferase assay | |||||||||||||||||||
| キーワード | ||||||||||||||||||||
| 主題Scheme | Other | |||||||||||||||||||
| 主題 | Trypanosoma congolense | |||||||||||||||||||
| 資源タイプ | ||||||||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||||
| 資源タイプ | journal article | |||||||||||||||||||
| 著者 |
Suganuma, Keisuke
× Suganuma, Keisuke
× Allamanda, Puttik
× Hakimi, Hassan
× Zhou, Mo
× Angeles, Jose Ma.
× Kawazu, Shin-ichiro
× Inoue, Noboru
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| 抄録 | ||||||||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||||||||
| 内容記述 | Animal African trypanosomosis (AAT), caused by Trypanosoma congolense, is widespread throughout sub-Saharan Africa. There are significant concerns related to the current drugs available for the treatment of AAT due to their limited effectiveness across species and their adverse effects. Moreover, drug resistant trypanosomes have recently been reported in the field. High throughput screening (HTS) of large chemical compound library collections is a promising approach for identifying novel drug candidates. While HTS for Trypanozoon trypanosomes, T. brucei sspp. and T. evansi is well established, no assays have been developed for T. congolense. In the present study, the authors developed an ATP-based luciferase viability assay for T. congolense in a 96-well plate format. The calculated 50% inhibitory concentration (IC50) values for pentamidine and diminazene were 10?100 times higher in T. congolense than in T. brucei. This result suggests that the transporters for the 2 tested compounds differ between T. congolense and T. brucei. This assay could further be applied to screen novel chemical compounds for the treatment of AAT caused by T. congolense | |||||||||||||||||||
| 書誌情報 |
Journal of Veterinary Medical Science 巻 76, 号 11, p. 1437-1441, 発行日 2014-12-01 |
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| 出版者 | ||||||||||||||||||||
| 出版者 | 日本獣医学会 | |||||||||||||||||||
| 出版者別言語 | ||||||||||||||||||||
| Japanese Society of Veterinary Science | ||||||||||||||||||||
| ISSN | ||||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||||
| 収録物識別子 | 09167250 | |||||||||||||||||||
| EISSN | ||||||||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||||||||
| 収録物識別子 | 13477439 | |||||||||||||||||||
| DOI | ||||||||||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||||||||||
| 識別子タイプ | DOI | |||||||||||||||||||
| 関連識別子 | 10.1292/jvms.14-0273 | |||||||||||||||||||
| 権利 | ||||||||||||||||||||
| 権利情報 | c 2014 by the Japanese Society of Veterinary Science | |||||||||||||||||||
| 著者版フラグ | ||||||||||||||||||||
| 出版タイプ | VoR | |||||||||||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||||||||||
| 引用 | ||||||||||||||||||||
| 内容記述タイプ | Other | |||||||||||||||||||
| 内容記述 | Journal of Veterinary Medical Science, 76(11), pp.1437-1441; 2014 | |||||||||||||||||||
