| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2014-07-01 |
| タイトル |
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|
タイトル |
HPLC Determination of Chlorpropamide in Human Serum by Fluorogenic Derivatization Based on the Suzuki Coupling Reaction with Phenylboronic Acid |
| 言語 |
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|
言語 |
eng |
| キーワード |
|
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主題Scheme |
Other |
|
主題 |
Chlorpropamide |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Fluorogenic derivatization |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
High-performance liquid chromatography |
| キーワード |
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主題Scheme |
Other |
|
主題 |
Phenylboronic acid |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Kishikawa, Naoya
Hammad, Sherin Farouk
Ohyama, Kaname
Kubo, Kimiko
Mabrouk, Mokhtar Mohamed
Nakashima, Kenichiro
Kuroda, Naotaka
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
A fluorogenic derivatization method for the determination of chlorpropamide in human serum was developed by means of high-performance liquid chromatography (HPLC) with fluorescence detection. The Suzuki coupling reaction with a non-fluorescent reagent, phenylboronic acid (PBA), was employed to convert chlorpropamide into highly fluorescent biphenyl derivative. Chlorpropamide was extracted from human serum by liquid-liquid extraction with toluene after addition of hydrochloric acid, and subsequently reacted with PBA. Because the fluorogenic derivatization was highly selective for aryl halide, the proposed method allowed sensitive and selective detection of chlorpropamide with a detection limit (at a signal to noise ratio of 3) of 0.5 ng mL-1. The sensitivity of our method was from 4 to 100 times better than HPLC-UV, gas chromatography, and LC-mass spectrometry. |
| 書誌情報 |
Chromatographia
巻 76,
号 11-12,
p. 703-706,
発行日 2013-06
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| 出版者 |
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出版者 |
Friedr. Vieweg und Sohn Verlags GmbH |
| ISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
00095893 |
| EISSN |
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
16121112 |
| DOI |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
10.1007/s10337-013-2451-5 |
| 権利 |
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|
権利情報 |
© 2013 Springer-Verlag Berlin Heidelberg. |
| 権利 |
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|
権利情報 |
The final publication is available at link.springer.com |
| 著者版フラグ |
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出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 引用 |
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|
内容記述タイプ |
Other |
|
内容記述 |
Chromatographia, 76(11-12), pp.703-706; 2013 |
Chr76_703.pdf (337.0 kB)
