| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-12-13 |
| タイトル |
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タイトル |
Functional Brachyury Binding Sites Establish a Temporal Read-out of Gene Expression in the Ciona Notochord |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
Katikala, Lavanya
Aihara, Hitoshi
Passamaneck, Yale J.
Gazdoiu, Stefan
José-Edwards, Diana S.
Kugler, Jamie E.
Oda-Ishii, Izumi
Imai, Janice H.
Nibu, Yutaka
Di, Gregorio Anna
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
The appearance of the notochord represented a milestone in Deuterostome evolution. The notochord is necessary for the development of the chordate body plan and for the formation of the vertebral column and numerous organs. It is known that the transcription factor Brachyury is required for notochord formation in all chordates, and that it controls transcription of a large number of target genes. However, studies of the structure of the cis-regulatory modules (CRMs) through which this control is exerted are complicated in vertebrates by the genomic complexity and the pan-mesodermal expression territory of Brachyury. We used the ascidian Ciona, in which the single-copy Brachyury is notochord-specific and CRMs are easily identifiable, to carry out a systematic characterization of Brachyury-downstream notochord CRMs. We found that Ciona Brachyury (Ci-Bra) controls most of its targets directly, through non-palindromic binding sites that function either synergistically or individually to activate early- and middle-onset genes, respectively, while late-onset target CRMs are controlled indirectly, via transcriptional intermediaries. These results illustrate how a transcriptional regulator can efficiently shape a shallow gene regulatory network into a multi-tiered transcriptional output, and provide insights into the mechanisms that establish temporal read-outs of gene expression in a fast-developing chordate embryo. |
| 書誌情報 |
PLoS Biology
巻 11,
号 10,
p. e1001697,
発行日 2013-10-29
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| 出版者 |
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出版者 |
Public Library of Science |
| EISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
15457885 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
10.1371/journal.pbio.1001697 |
| 権利 |
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権利情報 |
© 2013 Katikala et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 引用 |
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内容記述タイプ |
Other |
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内容記述 |
PLoS Biology, 11(10), e1001697; 2013 |
PLoS_Bio11_1001697.pdf (29.1 MB)
