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The Role of Fibronectin in Pulmonary Gene Transfer Following Intravenous Administration of Lipoplex in Mice
http://hdl.handle.net/10069/34049
http://hdl.handle.net/10069/34049dba11d87-f40b-4ee1-b57c-4af65a4fcb3c
名前 / ファイル | ライセンス | アクション |
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BPBul36_1807.pdf (573.8 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-01-09 | |||||
タイトル | ||||||
タイトル | The Role of Fibronectin in Pulmonary Gene Transfer Following Intravenous Administration of Lipoplex in Mice | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cationic liposome | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Fibronectin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Lipoplex | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Lung gene therapy | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Serum component | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Yoshikawa, Naoki
× Yoshikawa, Naoki× Fumoto, Shintaro× Nakashima, Mikiro× Shimokawa, Kenta× Miyamoto, Hirotaka× Nishida, Koyo |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We analyzed the effect of serum and fibronectin on pulmonary transgene expression after intravenous injection of cationic liposome-plasmid DNA (pDNA) complex (lipoplex) in mice. 1,2-Dioleoyl-3-trimethylammonium- propane (DOTAP) methyl sulfate salt/cholesterol lipoplex was incubated with several serum components for 5 min at 37°C prior to injection. We analyzed pulmonary transgene expression and pulmonary accumulation of lipoplex. While interaction with serum did not decrease pulmonary transgene expression, interaction with heat-inactivated serum did decrease it. Moreover, interaction with fibronectin enhanced pulmonary transgene expression. Inhibition of the binding of fibronectin to integrin decreased pulmonary transgene expression after injection of untreated lipoplex. We found that pulmonary accumulation of lipoplex changed depending on the kind of interacting serum components after injection. Furthermore, interaction with fibronectin increased pulmonary accumulation of lipoplex. Interaction with serum was required for pulmonary gene transfer following intravenous injection of lipoplex. Fibronectin appears to be a particularly critical component. Furthermore, the binding of fibronectin interacting with lipoplex to integrin was an important mechanism for pulmonary transgene expression. | |||||
書誌情報 |
Biological and Pharmaceutical Bulletin 巻 36, 号 11, p. 1807-1813, 発行日 2013-11-01 |
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出版者 | ||||||
出版者 | 日本薬学会 | |||||
出版者別言語 | ||||||
Pharmaceutical Society of Japan | ||||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 09186158 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13475215 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1248/bpb.b13-00500 | |||||
権利 | ||||||
権利情報 | © 2013 The Pharmaceutical Society of Japan. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Biological and Pharmaceutical Bulletin, 36(11), pp.1807-1813; 2013 |