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アマノリ葉体の生体凍結保存―1 : 海水中および半乾燥状態で凍結保存したアサクサノリ葉体の生存能力について
http://hdl.handle.net/10069/31576
http://hdl.handle.net/10069/3157615ae14a3-2aee-4152-af4e-40b99cc2b4bc
名前 / ファイル | ライセンス | アクション |
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suisan17_044.pdf (2.4 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2013-05-01 | |||||
タイトル | ||||||
タイトル | アマノリ葉体の生体凍結保存―1 : 海水中および半乾燥状態で凍結保存したアサクサノリ葉体の生存能力について | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
右田, 清治
× 右田, 清治 |
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著者別名 | ||||||
姓名 | Migita, Seiji | |||||
その他のタイトル | ||||||
その他のタイトル | Freeze-Preservation of Porphyra Thalli in Viable State―I.Viability of Porphyra tenera Preserved at Low Temperature after Freezing in the Sea Water and Freezing under Half-Dried Condition | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Some physiological studies on the freezing of algae had been reported. Recently many investigators have made researches on the preservation of micro-organisms for culture collections, and it seems likely that the freezing or freeze-drying is logical technique for maintainning viable cultures of cells, and has been successful in many instances. However, on marine algae, these experiments are rare in the literature. In this study, the author experimented on the freeze-preservation of thalli of Porphyra tenera and observed the viability of freeze-thawed thalli under the microscope. The results are summarized as follows: 1. When the cell of Porphyra thalli was injured by freezing, at first the plastid was scatterd, and then the cell expanded in size, finally shrinking up. 2. The vegetative and rhizoidal cells of thalli showed higher resistivity against freezing than the carposporangial cells. On the other hand, the survivals of neutral spores were more than those of carpospores and free living Chonchocelis-filaments. 3. From the results of freeze-preservation at various temperatures, the percentage of survival cells was higher, in freezing at -20℃ or over, than in deep-freezing at about -75℃. Furthermore, in deep freezing, the number of survival cells were less in rapid cooling than in slow cooling. 4. During freeze-preservation at -18~-20℃, most of cells frozen in the sea water were dead after 30 days storage, whereas many cells frozen under half-dried condition remained fully viable after 4 months. 5. The survivals of thalli frozen in the presence of glycerol did not increase, while glucose protected thalli against injury of freezepreservation. | |||||
書誌情報 |
長崎大学水産学部研究報告 巻 17, p. 44-54, 発行日 1964-09 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 05471427 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00178473 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
論文ID(NAID) | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | NAID | |||||
関連識別子 | 40018181174 | |||||
出版者 | ||||||
出版者 | 長崎大学水産学部 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 長崎大学水産学部研究報告, v.17, pp.44-54; 1964 |