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Salmonella enterotoxin (Stn) regulates membrane composition and integrity
http://hdl.handle.net/10069/29764
http://hdl.handle.net/10069/297641469f560-c317-4302-870a-27385a0813e5
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
DMM5_515.pdf (624.5 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2012-11-16 | |||||
| タイトル | ||||||
| タイトル | Salmonella enterotoxin (Stn) regulates membrane composition and integrity | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | cell membrane protein | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | CXCL1 chemokine | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | CXCL2 chemokine | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | CXCL3 chemokine | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | enterotoxin | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | granulocyte macrophage colony stimulating factor | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | monocyte chemotactic protein 3 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | outer membrane protein A | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | RANTES | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | salmonella enterotoxin | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | unclassified drug | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | amino acid sequence | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | animal experiment | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | article | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | bacterial membrane | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | bacterial strain | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | bacterial virulence | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | controlled study | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | female | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | gene deletion | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | human | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | human cell | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | immunogold labeling | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | in vitro study | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | in vivo study | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | mouse | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | nonhuman | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | phenotype | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | priority journal | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | protein interaction | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | protein localization | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | protein structure | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | proteomics | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Salmonella enterica | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | transmission electron microscopy | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Western blotting | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | wild type | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Nakano, Masayuki
× Nakano, Masayuki× Yamasaki, Eiki× Ichinose, Akitoyo× Shimohata, Takaaki× Takahashi, Akira× Akada, Junko K.× Nakamura, Kazuyuki× Moss, Joel× Hirayama, Toshiya× Kurazono, Hisao |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The mechanism of action of Salmonella enterotoxin (Stn) as a virulence factor in disease is controversial. Studies of Stn have indicated both positive and negative effects on Salmonella virulence. In this study, we attempted to evaluate Stn function and its effects on Salmonella virulence. To investigate Stn function, we first performed in vitro and in vivo analysis using mammalian cells and a murine ileal loop model. In these systems, we did not observe differences in virulence phenotypes between wild-type Salmonella and an stn gene-deleted mutant. We next characterized the phenotypes and molecular properties of the mutant strain under various in vitro conditions. The proteomic profiles of the total cell membrane protein fraction differed between wild type and mutant in that there was an absence of a protein in the mutant strain, which was identified as OmpA. By far-western blotting, OmpA was found to interact directly with Stn. To verify this result, the morphology of Salmonella was examined by transmission electron microscopy, with OmpA localization being analyzed by immunogold labeling. Compared with wild-type Salmonella, the mutant strain had a different pole structure and a thin periplasmic space; OmpA was not seen in the mutant. These results indicate that Stn, via regulation of OmpA membrane localization, functions in the maintenance of membrane composition and integrity. | |||||
| 書誌情報 |
Disease Models & Mechanisms 巻 5, 号 4, p. 515-521, 発行日 2012-07 |
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| 出版者 | ||||||
| 出版者 | Company of Biologists Ltd | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 17548403 | |||||
| EISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 17548411 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1242/dmm.009324 | |||||
| 権利 | ||||||
| 権利情報 | © 2012. Published by The Company of Biologists Ltd This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms |
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| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Disease Models & Mechanisms, 5(4), pp.515-521; 2012 | |||||
