WEKO3
アイテム
{"_buckets": {"deposit": "297dfab6-7b45-43a0-9ebb-ea190766b329"}, "_deposit": {"created_by": 2, "id": "9524", "owners": [2], "pid": {"revision_id": 0, "type": "depid", "value": "9524"}, "status": "published"}, "_oai": {"id": "oai:nagasaki-u.repo.nii.ac.jp:00009524", "sets": ["30"]}, "author_link": ["38458", "38463", "38460", "38452", "38455", "38454", "38461", "38453", "38456", "38457", "38459", "38462", "38451"], "item_2_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2012-07-26", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "7", "bibliographicPageStart": "e1002774", "bibliographicVolumeNumber": "8", "bibliographic_titles": [{"bibliographic_title": "PLoS Genetics"}]}]}, "item_2_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Two distinct Polycomb complexes, PRC1 and PRC2, collaborate to maintain epigenetic repression of key developmental loci in embryonic stem cells (ESCs). PRC1 and PRC2 have histone modifying activities, catalyzing mono-ubiquitination of histone H2A (H2AK119u1) and trimethylation of H3 lysine 27 (H3K27me3), respectively. Compared to H3K27me3, localization and the role of H2AK119u1 are not fully understood in ESCs. Here we present genome-wide H2AK119u1 maps in ESCs and identify a group of genes at which H2AK119u1 is deposited in a Ring1-dependent manner. These genes are a distinctive subset of genes with H3K27me3 enrichment and are the central targets of Polycomb silencing that are required to maintain ESC identity. We further show that the H2A ubiquitination activity of PRC1 is dispensable for its target binding and its activity to compact chromatin at Hox loci, but is indispensable for efficient repression of target genes and thereby ESC maintenance. These data demonstrate that multiple effector mechanisms including H2A ubiquitination and chromatin compaction combine to mediate PRC1-dependent repression of genes that are crucial for the maintenance of ESC identity. Utilization of these diverse effector mechanisms might provide a means to maintain a repressive state that is robust yet highly responsive to developmental cues during ES cell self-renewal and differentiation.", "subitem_description_type": "Abstract"}]}, "item_2_description_63": {"attribute_name": "引用", "attribute_value_mlt": [{"subitem_description": "PLoS Genetics, 8(7), e1002774; 2012", "subitem_description_type": "Other"}]}, "item_2_publisher_33": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Public Library of Science"}]}, "item_2_relation_12": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "10.1371/journal.pgen.1002774", "subitem_relation_type_select": "DOI"}}]}, "item_2_rights_13": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2012 Endoh et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]}, "item_2_source_id_8": {"attribute_name": "EISSN", "attribute_value_mlt": [{"subitem_source_identifier": "15537404", "subitem_source_identifier_type": "ISSN"}]}, "item_2_version_type_16": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Endoh, Mitsuhiro"}], "nameIdentifiers": [{"nameIdentifier": "38451", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Endo, Takaho A."}], "nameIdentifiers": [{"nameIdentifier": "38452", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Endoh, Tamie"}], "nameIdentifiers": [{"nameIdentifier": "38453", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Isono, Kyo-ichi"}], "nameIdentifiers": [{"nameIdentifier": "38454", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sharif, Jafar"}], "nameIdentifiers": [{"nameIdentifier": "38455", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ohara, Osamu"}], "nameIdentifiers": [{"nameIdentifier": "38456", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Toyoda, Tetsuro"}], "nameIdentifiers": [{"nameIdentifier": "38457", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ito, Takashi"}], "nameIdentifiers": [{"nameIdentifier": "38458", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Eskeland, Ragnhild"}], "nameIdentifiers": [{"nameIdentifier": "38459", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Bickmore, Wendy A."}], "nameIdentifiers": [{"nameIdentifier": "38460", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Vidal, Miguel"}], "nameIdentifiers": [{"nameIdentifier": "38461", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Bernstein, Bradley E."}], "nameIdentifiers": [{"nameIdentifier": "38462", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Koseki, Haruhiko"}], "nameIdentifiers": [{"nameIdentifier": "38463", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2020-12-22"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "PLoSGen8_1002774.pdf", "filesize": [{"value": "462.8 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 462800.0, "url": {"label": "PLoSGen8_1002774.pdf", "url": "https://nagasaki-u.repo.nii.ac.jp/record/9524/files/PLoSGen8_1002774.pdf"}, "version_id": "1c3a63a9-4a2a-4838-8bd1-ee37f0f4ae82"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Histone H2A Mono-Ubiquitination Is a Crucial Step to Mediate PRC1-Dependent Repression of Developmental Genes to Maintain ES Cell Identity", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Histone H2A Mono-Ubiquitination Is a Crucial Step to Mediate PRC1-Dependent Repression of Developmental Genes to Maintain ES Cell Identity"}]}, "item_type_id": "2", "owner": "2", "path": ["30"], "permalink_uri": "http://hdl.handle.net/10069/30158", "pubdate": {"attribute_name": "公開日", "attribute_value": "2013-01-04"}, "publish_date": "2013-01-04", "publish_status": "0", "recid": "9524", "relation": {}, "relation_version_is_last": true, "title": ["Histone H2A Mono-Ubiquitination Is a Crucial Step to Mediate PRC1-Dependent Repression of Developmental Genes to Maintain ES Cell Identity"], "weko_shared_id": -1}
Histone H2A Mono-Ubiquitination Is a Crucial Step to Mediate PRC1-Dependent Repression of Developmental Genes to Maintain ES Cell Identity
http://hdl.handle.net/10069/30158
http://hdl.handle.net/10069/30158fa0f077a-2076-4810-81f8-3d430ef1e9f4
名前 / ファイル | ライセンス | アクション |
---|---|---|
PLoSGen8_1002774.pdf (462.8 kB)
|
|
Item type | 学術雑誌論文 / Journal Article(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2013-01-04 | |||||
タイトル | ||||||
タイトル | Histone H2A Mono-Ubiquitination Is a Crucial Step to Mediate PRC1-Dependent Repression of Developmental Genes to Maintain ES Cell Identity | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Endoh, Mitsuhiro
× Endoh, Mitsuhiro× Endo, Takaho A.× Endoh, Tamie× Isono, Kyo-ichi× Sharif, Jafar× Ohara, Osamu× Toyoda, Tetsuro× Ito, Takashi× Eskeland, Ragnhild× Bickmore, Wendy A.× Vidal, Miguel× Bernstein, Bradley E.× Koseki, Haruhiko |
|||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Two distinct Polycomb complexes, PRC1 and PRC2, collaborate to maintain epigenetic repression of key developmental loci in embryonic stem cells (ESCs). PRC1 and PRC2 have histone modifying activities, catalyzing mono-ubiquitination of histone H2A (H2AK119u1) and trimethylation of H3 lysine 27 (H3K27me3), respectively. Compared to H3K27me3, localization and the role of H2AK119u1 are not fully understood in ESCs. Here we present genome-wide H2AK119u1 maps in ESCs and identify a group of genes at which H2AK119u1 is deposited in a Ring1-dependent manner. These genes are a distinctive subset of genes with H3K27me3 enrichment and are the central targets of Polycomb silencing that are required to maintain ESC identity. We further show that the H2A ubiquitination activity of PRC1 is dispensable for its target binding and its activity to compact chromatin at Hox loci, but is indispensable for efficient repression of target genes and thereby ESC maintenance. These data demonstrate that multiple effector mechanisms including H2A ubiquitination and chromatin compaction combine to mediate PRC1-dependent repression of genes that are crucial for the maintenance of ESC identity. Utilization of these diverse effector mechanisms might provide a means to maintain a repressive state that is robust yet highly responsive to developmental cues during ES cell self-renewal and differentiation. | |||||
書誌情報 |
PLoS Genetics 巻 8, 号 7, p. e1002774, 発行日 2012-07-26 |
|||||
出版者 | ||||||
出版者 | Public Library of Science | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 15537404 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1371/journal.pgen.1002774 | |||||
権利 | ||||||
権利情報 | © 2012 Endoh et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | PLoS Genetics, 8(7), e1002774; 2012 |