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Identification of a major glucose transporter in Flavobacterium johnsoniae: Inhibition of F. johnsoniae colony spreading by glucose uptake
http://hdl.handle.net/10069/38574
http://hdl.handle.net/10069/3857498884b18-d7f0-4588-8b8e-9d58e4ff2def
名前 / ファイル | ライセンス | アクション |
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MicImm62_507.pdf (1.3 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2019-09-01 | |||||
タイトル | ||||||
タイトル | Identification of a major glucose transporter in Flavobacterium johnsoniae: Inhibition of F. johnsoniae colony spreading by glucose uptake | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | colony spreading | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | gliding motility | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | glucose uptake | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | major facilitator superfamily transporter | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Imamura, Keigo
× Imamura, Keigo× Sato, Keiko× Narita, Yuka× Kondo, Yoshio× Nakane, Daisuke× Naito, Mariko× Fujiwara, Taku× Nakayama, Koji |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Many members of the phylum Bacteroidetes, such as Flavobacterium johnsoniae, can glide over a solid surface: an ability called gliding motility. It can be usually observed on agar plates as thin, flat, spreading colonies with irregular, feathery edges; this phenomenon is called colony spreading. Colony spreading of F. johnsoniae on 1.5% agar plates containing poor nutrients is dose-dependently inhibited by addition of D-glucose, as previously reported. Accordingly, here, we created mutants (by transposon mutagenesis) that partially suppressed glucose-mediated inhibition of colony spreading. Among the isolates, we found that one had a transposon insertion in Fjoh_4565, tentatively named mfsA, which encodes a major facilitator superfamily (MFS) transporter previously shown to be required for growth on glucose, N-acetyl-glucosamine, and chitin. We constructed an mfsA deletion mutant and found that the mutant showed no glucose-mediated acceleration of growth or glucose uptake. The mfsA gene complemented the phenotype of a glucose-negative Escherichia coli. These results suggest that the mfsA gene encodes the sole MFS transporter of glucose in F. johnsoniae and that glucose uptake is partially required for the glucose-mediated inhibition of F. johnsoniae colony spreading. | |||||
書誌情報 |
Microbiology and Immunology 巻 62, 号 8, p. 507-516, 発行日 2018-08 |
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出版者 | ||||||
出版者 | John Wiley & Sons Australia, Ltd | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 03855600 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1111/1348-0421.12633 | |||||
権利 | ||||||
権利情報 | c 2018 The Societies and John Wiley & Sons Australia, Ltd. This is the peer reviewed version of the following article:Microbiology and Immunology, 62(8), pp.507-516; 2018, which has been published in final form at http://dx.doi.org/10.1111/1348-0421.12633 .This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Microbiology and Immunology, 62(8), pp.507-516; 2018 |