Multiplex PCR analysis of HPRT(-) mutations in human embryo (HE) cells induced by 230 keV/μm carbon-ion irradiation showed no large deletion around the exon regions of the locus gene in contrast to the irradiations at different LETs. To identify these mutations, the sequence alterations in a cDNA of hprt gene were determined for 18 mutant clones in this study. Missing of exon 6 was the most frequent mutational event (10 clones), and missing of both exons 6 and 8 was next most frequent event (6 clones), then base substitutions (2 clones). These characteristics were not seen in a similar analysis of spontaneous mutations, which showed base substitution (5 clones), frameshift (2 clones), missing of both exons 2 and 3 (2 clones), and a single unidentified clone. Direct sequencing and restriction enzyme digestion of the genomic DNA of the mutants which showed missing of exons 6 and 8 in the cDNA, supports the possibility that they were induced by aberrant mRNA splicing.
雑誌名
Journal of radiation research
巻
36
号
3
ページ
185 - 195
発行年
1995-09
出版者
日本放射線影響学会
ISSN
04493060
書誌レコードID
AA00705792
権利
日本放射線影響学会
本文データは学協会の許諾に基づきCiNiiから複製したものである
著者版フラグ
publisher
引用
Journal of radiation research. 1995, 36(3), p.185-195
Analysis of Mutations in the Human HPRT Gene Induced by Accelerated Heavy-Ion Irradiation
RR36_3_185_195.pdf
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