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Simple and Sensitive Enzyme-Linked Immunosorbent Assay for Ivermectin
http://hdl.handle.net/10069/21861
http://hdl.handle.net/10069/21861d76aeff2-3377-434d-9509-41b632b29d4a
名前 / ファイル | ライセンス | アクション |
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ajtmh54_243.pdf (537.3 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2009-05-13 | |||||
タイトル | ||||||
タイトル | Simple and Sensitive Enzyme-Linked Immunosorbent Assay for Ivermectin | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Mitsui, Yoshinori
× Mitsui, Yoshinori× Tanimori, Hideaki× Kitagawa, Tsunehiro× Fujimaki, Yasunori× Aoki, Yoshiki |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A sensitive and reproducible enzyme-linked immunosorbent assay (ELISA) for the determination of the concentration of ivermectin (IVM) in biological fluids was developed. A conjugate of IVM on bovine serum albumin and poly-L-lysine was used to produce antibodies in rabbits and served as a solid-phase marker for titration of antibodies, respectively. The competitive ELISA was conducted by simultaneously incubating IVM and IVM-biotin conjugate with anti-IVM antiserum over goat anti-rabbit IgG (Fc) and then determining the amount of bound IVM-biotin with avidin-peroxidase conjugate as a tracer. The coefficient of variation for the assay was less than 10% in the range of 0.3?10 ng/ml. The limit of detection was 0.1 ng/ml. The cross-reactivities of anti-IVM antiserum with some anthelmintic drugs were negligible. Using this ELISA, serum levels of IVM were easily determined in Mongolian jirds (Meriones unguiculatus) up to 72 hr following a single oral dose of 500 μg/kg of body weight. | |||||
書誌情報 |
American Journal of Tropical Medicine and Hygiene 巻 54, 号 3, p. 243-248, 発行日 1996-03 |
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出版者 | ||||||
出版者 | American Society of Tropical Medicine and Hygiene | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00029637 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00521439 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | 8600758 | |||||
権利 | ||||||
権利情報 | Copyright c 1996 by The American Society of Tropical Medicine and Hygiene | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
関係URI | ||||||
関連名称 | http://www.ajtmh.org/cgi/content/abstract/54/3/243 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | American Journal of Tropical Medicine and Hygiene, 54(3), pp.243-248; 1996 |