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Analysis of Mutations in the Human HPRT Gene Induced by Accelerated Heavy-Ion Irradiation
http://hdl.handle.net/10069/18734
http://hdl.handle.net/10069/187344686930e-983c-4ee5-8f34-1ea0d0e685b8
名前 / ファイル | ライセンス | アクション |
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RR36_3_185_195.pdf (494.7 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-07-24 | |||||
タイトル | ||||||
タイトル | Analysis of Mutations in the Human HPRT Gene Induced by Accelerated Heavy-Ion Irradiation | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Mutation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | HPRT | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Heavy-Ion Irradiation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Aberrant mRNA Splicing | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | High LET | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Kagawa, Yasuhiro
× Kagawa, Yasuhiro× Yatagai, Fumio× Suzuki, Masao× Kase, Youko× Kobayashi, Akiko× Hirano, Masahiko× Kato, Takesi× Watanabe, Masami× Hanaoka, Fumio |
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著者別名 | ||||||
姓名 | 谷田貝, 文夫 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Multiplex PCR analysis of HPRT(-) mutations in human embryo (HE) cells induced by 230 keV/μm carbon-ion irradiation showed no large deletion around the exon regions of the locus gene in contrast to the irradiations at different LETs. To identify these mutations, the sequence alterations in a cDNA of hprt gene were determined for 18 mutant clones in this study. Missing of exon 6 was the most frequent mutational event (10 clones), and missing of both exons 6 and 8 was next most frequent event (6 clones), then base substitutions (2 clones). These characteristics were not seen in a similar analysis of spontaneous mutations, which showed base substitution (5 clones), frameshift (2 clones), missing of both exons 2 and 3 (2 clones), and a single unidentified clone. Direct sequencing and restriction enzyme digestion of the genomic DNA of the mutants which showed missing of exons 6 and 8 in the cDNA, supports the possibility that they were induced by aberrant mRNA splicing. | |||||
書誌情報 |
Journal of radiation research 巻 36, 号 3, p. 185-195, 発行日 1995-09 |
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出版者 | ||||||
出版者 | 日本放射線影響学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 04493060 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00705792 | |||||
権利 | ||||||
権利情報 | 日本放射線影響学会 | |||||
権利 | ||||||
権利情報 | 本文データは学協会の許諾に基づきCiNiiから複製したものである | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of radiation research. 1995, 36(3), p.185-195 |