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Development and evaluation of a rapid and simple diagnostic assay for COVID-19 based on loop-mediated isothermal amplification
http://hdl.handle.net/10069/40425
http://hdl.handle.net/10069/404253a7f3026-3b9b-427b-8950-7eb36b41113d
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
PloSNeg14_8855.pdf (825.8 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2020-12-04 | |||||
| タイトル | ||||||
| タイトル | Development and evaluation of a rapid and simple diagnostic assay for COVID-19 based on loop-mediated isothermal amplification | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Yoshikawa, Rokusuke
× Yoshikawa, Rokusuke× Abe, Haruka× Igasaki, Yui× Negishi, Saeki× Goto, Hiroaki× Yasuda, Jiro |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a highly pathogenic novel coronavirus that has caused a worldwide outbreak.Here we describe a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay that uses a portable device for efficient detection of SARS-CoV-2. This RT-LAMP assay specifically detected SARS-CoV-2 without cross-reacting with the most closely related human coronavirus, SARS-CoV. Clinical evaluation of nasal swab samples from suspected SARS-CoV-2 pneumonia (COVID-19) patients showed that the assay could detect over 23.7 copies within 15 min with a 100% probability. Since the RT-LAMP assay can be performed with a portable battery-supported device, it is a rapid, simple, and sensitive diagnostic assay for COVID-19 that can be available at point-of-care. We also developed the RT-LAMP assay without the RNA extraction step-Direct RT-LAMP, which could detect more than 1.43 x 103 copies within 15 min with a 100% probability in clinical evaluation test. Although the Direct RT-LAMP assay was less sensitive than the standard RT-LAMP, the Direct RT-LAMP assay can be available as the rapid first screening of COVID-19 in poorly equipped areas, such as rural areas in developing countries. |
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| 書誌情報 |
PLOS Neglected Tropical Diseases 巻 14, 号 11, p. e0008855, 発行日 2020-11-04 |
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| 出版者 | ||||||
| 出版者 | Public Library of Science | |||||
| EISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 19352735 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1371/journal.pntd.0008855 | |||||
| 権利 | ||||||
| 権利情報 | c 2020 Yoshikawa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | PLoS neglected tropical diseases, 14(11), art.no.e0008855; 2020 | |||||
