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Toxicoproteomic analysis of a mouse model of nonsteroidal anti-inflammatory drug-induced gastric ulcers
http://hdl.handle.net/10069/29072
http://hdl.handle.net/10069/2907243cc92d1-be56-4635-bacb-fe8a909965da
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
BBRC420_210.pdf (560.0 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2012-08-24 | |||||
| タイトル | ||||||
| タイトル | Toxicoproteomic analysis of a mouse model of nonsteroidal anti-inflammatory drug-induced gastric ulcers | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Fluorogenic derivatization-liquid chromatography tandem mass spectrometry | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Gastric ulceration | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Nonsteroidal anti-inflammatory drugs | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Toxicoproteomics | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Ohyama, Kaname
× Ohyama, Kaname× Shiokawa, Akina× Ito, Kosei× Masuyama, Ritsuko× Ichibangase, Tomoko× Kishikawa, Naoya× Imai, Kazuhiro× Kuroda, Naotaka |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Nonsteroidal anti-inflammatory drugs (NSAIDs) are valuable agents; however, their use has been limited by their association with mucosal damage in the upper gastrointestinal tract. NSAIDs inhibit cyclooxygenase and consequently block the synthesis of prostaglandins, which have cytoprotective effects in gastric mucosa; these effects on prostaglandins have been thought to be major cause of NSAID-induced ulceration. However, studies indicate that additional NSAID-related mechanisms are involved in formation of gastric lesions. Here, we used a toxicoproteomic approach to understand cellular processes that are affected by NSAIDs in mouse stomach tissue during ulcer formation. We used fluorogenic derivatization-liquid chromatography-tandem mass spectrometry (FD-LC-MS/MS)-which consists of fluorogenic derivatization, separation and fluorescence detection by LC, and identification by LC-tandem mass spectrometry-in this proteomic analysis of pyrolic stomach from control and diclofenac (Dic)-treated mice. FD-LC-MS/MS results were highly sensitive; 10 differentially expressed proteins were identified, and all 10 were more highly expressed in Dic-treated mice than in control mice. Specifically, expression levels of 78. kDa glucose-regulated protein (GRP78), heat shock protein beta-1 (HSP27), and gastrin were more than 3-fold higher in Dic-treated mice than in control mice. This study represents a first step to ascertain the precise actors of early NSAID-induced ulceration. | |||||
| 書誌情報 |
Biochemical and Biophysical Research Communications 巻 420, 号 1, p. 210-215, 発行日 2012-03-30 |
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| 出版者 | ||||||
| 出版者 | Elsevier Inc. | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0006291X | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1016/j.bbrc.2012.03.009 | |||||
| 権利 | ||||||
| 権利情報 | © 2012 Elsevier Inc. | |||||
| 権利 | ||||||
| 権利情報 | NOTICE: this is the author’s version of a work that was accepted for publication in Biochemical and Biophysical Research Communications. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Biochemical and Biophysical Research Communications, 420, 1(2012) | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Biochemical and Biophysical Research Communications, 420(1), pp.210-215; 2012 | |||||
