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A novel carbohydrate-binding surface layer protein from the hyperthermophilic archaeon Pyrococcus horikoshii
http://hdl.handle.net/10069/38598
http://hdl.handle.net/10069/38598552261ba-a892-49c1-b748-3f9cb2933620
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
BBB82_1327.pdf (492.1 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2019-04-08 | |||||
| タイトル | ||||||
| タイトル | A novel carbohydrate-binding surface layer protein from the hyperthermophilic archaeon Pyrococcus horikoshii | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Archaea | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Carbohydrate binding | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Pyrococcus horikoshii | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Surface-layer protein | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Goda, Shuichiro
× Goda, Shuichiro× Koga, Tomoyuki× Yamashita, Kenichiro× Kuriura, Ryo× Ueda, Toshifumi |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In Archaea and Bacteria, surface layer (S-layer) proteins form the cell envelope and are involved in cell protection. In the present study, a putative S-layer protein was purified from the crude extract of Pyrococcus horikoshii using affinity chromatography. The S-layer gene was cloned and expressed in Escherichia coli. Isothermal titration calorimetry analyses showed that the S-layer protein bound N- acetylglucosamine and induced agglutination of the gram-positive bacterium Micrococcus lysodeikticus. The protein comprised a 21-mer structure, with a molecular mass of 1,340 kDa, as determined using small-angle X-ray scattering. This protein showed high thermal stability, with a midpoint of thermal denaturation of 79 °C in dynamic light scattering experiments. This is the first description of the carbohydrate-binding archaeal S-layer protein and its characteristics. |
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| 書誌情報 |
Bioscience, Biotechnology, and Biochemistry 巻 82, 号 8, p. 1327-1334, 発行日 2018-04-08 |
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| 出版者 | ||||||
| 出版者 | 日本農芸化学会 | |||||
| 出版者別言語 | ||||||
| 値 | Japan Society for Bioscience, Biotechnology, and Agrochemistry | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 09168451 | |||||
| EISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 13476947 | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1080/09168451.2018.1460571 | |||||
| 権利 | ||||||
| 権利情報 | c 2018 Japan society for Bioscience, Biotechnology, and Agrochemistry. This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience, Biotechnology and Biochemistry on 08 Apr 2018, available online: https://www.tandfonline.com/doi/abs/10.1080/09168451.2018.1460571 | |||||
| 著者版フラグ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Bioscience, Biotechnology and Biochemistry, 82(8), pp.1327-1334; 2018 | |||||
