WEKO3
アイテム
iPS cell serves as a source of dendritic cells for in vitro dengue virus infection model
http://hdl.handle.net/10069/38627
http://hdl.handle.net/10069/3862780e47336-f749-4dd8-bce7-5381e0cff7d8
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
ISYK1094_Dao.pdf (2.7 MB)
|
|
| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2018-10-18 | |||||
| タイトル | ||||||
| タイトル | iPS cell serves as a source of dendritic cells for in vitro dengue virus infection model | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | in vitro model | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | cellular immunity | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | dendritic cell | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | iPS cell | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | antigen presentation | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | dengue virus | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
| 資源タイプ | doctoral thesis | |||||
| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 著者 |
Dao, Huy Manh
× Dao, Huy Manh |
|||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 116198 | |||||
| 姓名 | ダオ, フイ マン | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The lack of an appropriate model has been a serious concern in dengue research pertinent to immune response and vaccine development. It remains a matter of impediment in dengue virus (DENV) studies when it comes to an in vitro model, which requires adequate quantity of dendritic cells (DC) with uniform characters. Other sources of DC, mostly monocyte derived DC (moDC), have been used despite their limitations such as quantity, proliferation, and donor dependent characters. Recent development of human iPS cells with consistent proliferation for long, stable, functional characteristics and desired HLA background has certainly offered added advantages. Therefore, we hypothesised that iPS derived cells would be a reliable alternative to the traditional DCs to be used with an in vitro DENV system. To develop a DENV infection and T cell activation model, we utilised iPS cells (HLA-A*24) as the source of DC. iPS-ML-DC was prepared and DENV infectivity was assessed apart from the major surface markers expression and cytokine production potential. Our iPS-ML-DC had major DC markers expression, DENV infection efficiency and cytokine production properties similar to that of moDC. Moreover, DENV infected iPS-ML-DC demonstrated the ability to activate HLA-matched T cell (but not mismatched) in vitro as evidenced by significantly higher proportion of IFN-γ+ CD69+ T cells compared to non-infected iPS-ML-DC. This affirmed the antigen-specific T cell activation by iPS-ML-DC as a function of antigen presenting cells. To conclude, maturation potential, DENV infection efficiency and T cell activation ability collectively suggest that iPS-ML-DC serves as an attractive option of DC for use in DENV studies in vitro. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 長崎大学学位論文 学位記番号:博(医歯薬)甲第1094号 学位授与年月日:平成30年9月5日 | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Author: Dao Huy Manh, Shusaku Mizukami, Shyam Prakash Dumre, Muhareva Raekiansyah, Satoru Senju, Yasuharu Nishimura, Juntra Karbwang, Nguyen Tien Huy, Kouichi Morita, Kenji Hirayama | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Citation: Journal of General Virology, 99(9), pp.1239-1247; 2018 | |||||
| 書誌情報 |
Journal of General Virology 巻 99, 号 9, p. 1239-1247, 発行日 2018-09-05 |
|||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00221317 | |||||
| EISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 14652099 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | 10.1099/jgv.0.001119 | |||||
| 権利 | ||||||
| 権利情報 | c 2018 The Authors. Published by the Microbiology Society. | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| その他のタイトル | ||||||
| その他のタイトル | iPS細胞はin vitroデングウイルス感染実験に用いられる樹状細胞の由来として有用である | |||||
| 出版者 | ||||||
| 出版者 | Microbiology Society | |||||
| 学位名 | ||||||
| 学位名 | 博士(医学) | |||||
| 学位授与機関 | ||||||
| 学位授与機関識別子Scheme | kakenhi | |||||
| 学位授与機関識別子 | 17301 | |||||
| 学位授与機関名 | Nagasaki University (長崎大学) | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2018-09-05 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 甲医歯薬第1094号 | |||||
| 学位の種類 | ||||||
| 値 | 課程博士 | |||||
| 引用 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Nagasaki University (長崎大学), 博士(医学) (2018-09-05) | |||||
