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A semi-automated non-radioactive system for measuring recovery of RNA synthesis and unscheduled DNA synthesis using ethynyluracil derivatives.
http://hdl.handle.net/10069/23067
http://hdl.handle.net/10069/23067a38b6dca-37f4-41b9-879d-f52706f6baf2
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||
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公開日 | 2010-03-15 | |||||||||||||
タイトル | ||||||||||||||
タイトル | A semi-automated non-radioactive system for measuring recovery of RNA synthesis and unscheduled DNA synthesis using ethynyluracil derivatives. | |||||||||||||
言語 | ||||||||||||||
言語 | eng | |||||||||||||
キーワード | ||||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Cockayne syndrome (CS) | |||||||||||||
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主題Scheme | Other | |||||||||||||
主題 | Nucleotide excision repair (NER) | |||||||||||||
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主題Scheme | Other | |||||||||||||
主題 | Recovery of RNA synthesis (RRS) | |||||||||||||
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主題Scheme | Other | |||||||||||||
主題 | Transcription-coupled repair (TCR) | |||||||||||||
キーワード | ||||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Unscheduled DNA synthesis (UDS) | |||||||||||||
キーワード | ||||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Xeroderma pigmentosum (XP) | |||||||||||||
資源タイプ | ||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
資源タイプ | journal article | |||||||||||||
著者 |
Nakazawa, Yuka
× Nakazawa, Yuka
× Yamashita, Shunichi
× Lehmann, Alan R
× Ogi, Tomoo
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抄録 | ||||||||||||||
内容記述タイプ | Abstract | |||||||||||||
内容記述 | Nucleotide excision repair (NER) removes the major UV-photolesions from cellular DNA. In humans, compromised NER activity is the cause of several photosensitive diseases, one of which is the skin-cancer predisposition disorder, xeroderma pigmentosum (XP). Two assays commonly used in measurement of NER activity are 'unscheduled DNA synthesis (UDS)', and 'recovery of RNA synthesis (RRS)', the latter being a specific measure of the transcription-coupled repair sub-pathway of NER. Both assays are key techniques for research in NER as well as in diagnoses of NER-related disorders. Until very recently, reliable methods for these assays involved measurements of incorporation of radio-labeled nucleosides. We have established non-radioactive procedures for determining UDS and RRS levels by incorporation of recently developed alkyne-conjugated nucleoside analogues, 5-ethynyl-2'-deoxyuridine (EdU) and 5-ethynyuridine (EU). EdU and EU are respectively used as alternatives for (3)H-thymidine in UDS and for (3)H-uridine in RRS. Based on these alkyne-nucleosides and an integrated image analyser, we have developed a semi-automated assay system for NER-activity. We demonstrate the utility of this system for NER-activity assessments of lymphoblastoid samples as well as primary fibroblasts. Potential use of the system for large-scale siRNA-screening for novel NER defects as well as for routine XP diagnosis are also considered. | |||||||||||||
書誌情報 |
DNA repair 巻 9, 号 5, p. 506-516, 発行日 2010-05-04 |
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出版者 | ||||||||||||||
出版者 | Elsevier B.V. | |||||||||||||
ISSN | ||||||||||||||
収録物識別子タイプ | ISSN | |||||||||||||
収録物識別子 | 15687864 | |||||||||||||
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収録物識別子タイプ | ISSN | |||||||||||||
収録物識別子 | 15687856 | |||||||||||||
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収録物識別子タイプ | NCID | |||||||||||||
収録物識別子 | AA11032547 | |||||||||||||
PubMed番号 | ||||||||||||||
関連タイプ | isVersionOf | |||||||||||||
識別子タイプ | PMID | |||||||||||||
関連識別子 | 20171149 | |||||||||||||
DOI | ||||||||||||||
関連タイプ | isVersionOf | |||||||||||||
識別子タイプ | DOI | |||||||||||||
関連識別子 | 10.1016/j.dnarep.2010.01.015 | |||||||||||||
権利 | ||||||||||||||
権利情報 | Copyright © 2010 Elsevier B.V. All rights reserved. | |||||||||||||
著者版フラグ | ||||||||||||||
出版タイプ | AM | |||||||||||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||
引用 | ||||||||||||||
内容記述タイプ | Other | |||||||||||||
内容記述 | DNA repair, 9(5), pp.506-516; 2010 |