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Determination of active site of lysine-specific cysteine proteinase (Lys-gingipain) by use of a Porphyromonas gingivalis plasmid system.
http://hdl.handle.net/10069/23114
http://hdl.handle.net/10069/231142ff8da78-bfcf-4a3e-aeee-e7a185f17fda
名前 / ファイル | ライセンス | アクション |
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AOB53_538.pdf (1.0 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-04-15 | |||||
タイトル | ||||||
タイトル | Determination of active site of lysine-specific cysteine proteinase (Lys-gingipain) by use of a Porphyromonas gingivalis plasmid system. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Arg-gingipain | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Lys-gingipain | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Periodontal diseases | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Porphyromonas gingivalis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Recombinant expression | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Ishida, Yutaka
× Ishida, Yutaka× Hu, Jinping× Sakai, Eiko× Kadowaki, Tomoko× Yamamoto, Kenji× Tsukuba, Takayuki× Kato, Yuzo× Nakayama, Koji× Okamoto, Kuniaki |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Porphyromonas gingivalis, a major etiological bacterium of periodontal diseases, produces a unique lysine-specific cysteine proteinase (Lys-gingipain, Kgp) implicated in the virulence of this organism. Our observations show the expression of a catalytically active recombinant Kgp in a P. gingivalis Kgp-null mutant and the restoration of its functions by the use of a shuttle plasmid vector stable in P. gingivalis. The Kgp-expressing mutant exhibited a similar catalytic activity to that of the wild-type strain. This mutant also restored the ability to form black-pigmented colonies on blood agar plates and to generate a 19-kDa haemoglobin receptor protein responsible for haemoglobin binding. In order to establish the importance of the active-site Cys residue and elucidate its role in bacterial black pigmentation we constructed three Kgp mutants with changed potential active-site Cys residues. The cells expressing a single mutation (C476A) showed the high Kgp activity and the black pigmentation. In contrast, the cells expressing the single mutant (C477A) and the double mutant (C476A/C477A) exhibited neither Kgp activity nor black pigmentation. These results indicate that the 477th Cys residue is essential for both the Kgp activity and the black pigmentation of P. gingivalis. | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 長崎大学学位論文 学位記番号:博(医歯薬)甲第139号 学位授与年月日:平成20年2月27日 | |||||
書誌情報 |
Archives of oral biology 巻 53, 号 6, p. 538-544, 発行日 2008-06 |
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出版者 | ||||||
出版者 | Elsevier Ltd | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00039969 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00548457 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 18295742 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.archoralbio.2008.01.004 | |||||
権利 | ||||||
権利情報 | Copyright © 2008 Elsevier Ltd All rights reserved. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
関係URI | ||||||
関連名称 | http://hdl.handle.net/10069/16965 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Archives of oral biology, 53(6), pp.538-544; 2008 |