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Evaluation of chemiluminescence reagents for selective detection of reactive oxygen species.
http://hdl.handle.net/10069/23180
http://hdl.handle.net/10069/23180941dc277-b314-4fe0-b86a-8b9a29d91440
名前 / ファイル | ライセンス | アクション |
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ACA665_74.pdf (162.4 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-06-01 | |||||
タイトル | ||||||
タイトル | Evaluation of chemiluminescence reagents for selective detection of reactive oxygen species. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Acridinium ester | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Chemiluminescent probe | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Reactive oxygen species | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Screening | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Superoxide dismutase | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Yamaguchi, Shinya
× Yamaguchi, Shinya× Kishikawa, Naoya× Ohyama, Kaname× Ohba, Yoshihito× Kohno, Maiko× Masuda, Toshinobu× Takadate, Akira× Nakashima, Kenichiro× Kuroda, Naotaka |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | In order to evaluate the chemiluminescence (CL) reagents for selective detection of reactive oxygen species (ROS), we comprehensively measured the CL responses of 20 CL reagents (three luminol derivatives, two imidazopyrazinone derivatives, eight lophine derivatives, six acridinium ester derivatives and lucigenin) against six types of ROS (superoxide anion: O(2)(*-), hydroxyl radical: *OH, hydrogen peroxide: H(2)O(2), hypochlorite anion: ClO(-), singlet oxygen: (1)O(2), and nitric oxide: NO). As a result of the screening, it was found that nine CL reagents selectively detected O(2)(*-) while one CL reagent selectively detected *OH. However, no CL reagent had selectivity on the detection of H(2)O(2), ClO(-), (1)O(2) and NO. Our screening results could help to select the most suitable CL reagent for selective determination of different ROS. As an application study, 4-methoxyphenyl-10-methylacridinium-9-carboxylate (MMAC), one of the acridinium ester derivatives, showed high selectivity on the detection of O(2)(*-), and thus was applied to the assay of superoxide dismutase (SOD) activity. The dynamic range and detection limit of the developed CL assay were 0.1-10 and 0.06 U mL(-1), respectively. Significant correlation (r=0.997) was observed between the results by the CL assay using MMAC and the spectrophotometric assay using 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium monosodium salt. | |||||
書誌情報 |
Analytica chimica acta 巻 665, 号 1, p. 74-78, 発行日 2010-04-14 |
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出版者 | ||||||
出版者 | Elsevier B.V. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00032670 | |||||
EISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 18734324 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00524834 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 20381693 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.aca.2010.03.025 | |||||
権利 | ||||||
権利情報 | Crown copyright © 2010 Published by Elsevier B.V. | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Analytica chimica acta, 665(1), pp.74-78; 2010 |