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A novel and simple micro-irradiation technique for creating localized DNA double-strand breaks.
http://hdl.handle.net/10069/23875
http://hdl.handle.net/10069/23875981c9fab-4d63-420d-b764-3e880393cc38
名前 / ファイル | ライセンス | アクション |
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NAR38_129.pdf (4.5 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2010-09-10 | |||||
タイトル | ||||||
タイトル | A novel and simple micro-irradiation technique for creating localized DNA double-strand breaks. | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Suzuki, Keiji
× Suzuki, Keiji× Yamauchi, Motohiro× Oka, Yasuyoshi× Suzuki, Masatoshi× Yamashita, Shunichi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | An ataxia-telangiectasia mutated (ATM)-dependent DNA damage signal is amplified through the interaction of various factors, which are recruited to the chromatin regions with DNA double-strand breaks. Spatial and temporal regulation of such factors is analysed by fluorescence microscopy in combination with laser micro-irradiation. Here we describe a novel and simple technique for micro-irradiation that does not require a laser source. Cells were labelled with BrdU for 48-72 h, covered with porous polycarbonate membranes, and exposed to UVC. All BrdU-labelled cells showed localized foci of phosphorylated ATM, phosphorylated histone H2AX, MDC1 and 53BP1 upon irradiation, showing that these foci were induced irrespective of the cell-cycle phase. They were also detectable in nucleotide excision repair-defective XPA cells labelled with BrdU, indicating that the foci did not reflect an excision repair-related process. Furthermore, an ATM-specific inhibitor significantly attenuated the foci formation, and disappearance of the foci was significantly abrogated in non-homologous end-joining-defective cells. Thus, it can be concluded that micro-irradiation generated DNA double-strand breaks in BrdU-sensitized cells. The present technique should accelerate research in the fields of DNA damage response, DNA repair and DNA recombination, as it provides more chances to perform micro-irradiation experiments without any specific equipment. | |||||
書誌情報 |
Nucleic acids research 巻 38, 号 12, p. e129-e129, 発行日 2010-07-01 |
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出版者 | ||||||
出版者 | Oxford University Press | |||||
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収録物識別子タイプ | ISSN | |||||
収録物識別子 | 13624962 | |||||
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収録物識別子タイプ | NCID | |||||
収録物識別子 | AA1203820X | |||||
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関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | 20385602 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1093/nar/gkq226 | |||||
権利 | ||||||
権利情報 | © The Author(s) 2010. Published by Oxford University Press. | |||||
権利 | ||||||
権利情報 | This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
引用 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Nucleic acids research, 38(12), pp.e129; 2010 |