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Negative pressure induces dedifferentiation of hepatocytes via RhoA/ROCK pathway
http://hdl.handle.net/10069/0002000036
http://hdl.handle.net/10069/0002000036b75e2c26-7c48-432c-b76f-a3db372e060a
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||||||
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公開日 | 2023-07-13 | |||||||||||||||||
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言語 | en | |||||||||||||||||
タイトル | Negative pressure induces dedifferentiation of hepatocytes via RhoA/ROCK pathway | |||||||||||||||||
言語 | ||||||||||||||||||
言語 | eng | |||||||||||||||||
キーワード | ||||||||||||||||||
言語 | en | |||||||||||||||||
主題Scheme | Other | |||||||||||||||||
主題 | Hepatocytes | |||||||||||||||||
キーワード | ||||||||||||||||||
言語 | en | |||||||||||||||||
主題Scheme | Other | |||||||||||||||||
主題 | Dedifferentiation | |||||||||||||||||
キーワード | ||||||||||||||||||
言語 | en | |||||||||||||||||
主題Scheme | Other | |||||||||||||||||
主題 | Stemness | |||||||||||||||||
キーワード | ||||||||||||||||||
言語 | en | |||||||||||||||||
主題Scheme | Other | |||||||||||||||||
主題 | RhoA/ROCK | |||||||||||||||||
キーワード | ||||||||||||||||||
言語 | en | |||||||||||||||||
主題Scheme | Other | |||||||||||||||||
主題 | Negative pressure | |||||||||||||||||
資源タイプ | ||||||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||||||
資源タイプ | journal article | |||||||||||||||||
著者 |
Khalifa, Mahmoud Osman
× Khalifa, Mahmoud Osman
× Moriwaki, Takahito
× Zhang, Shouhua
× Zhou, Wei
× Ito, Kosei
× Li, Tao-Sheng
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抄録 | ||||||||||||||||||
内容記述タイプ | Abstract | |||||||||||||||||
内容記述 | Biomechanical forces are known to regulate the biological behaviors of cells. Although negative pressure has been used for wound healing, it is still unknown about its role in regulating cell plasticity. We investigated whether negative pressure could induce the dedifferentiation of hepatocytes. Using a commercial device, we found that the exposure of primary human hepatocytes to −50 mmHg quickly induced the formation of stress fibers and obviously changed cell morphology in 72 h. Moreover, the exposure of hepatocytes to −50 mmHg significantly upregulated RhoA, ROCK1, and ROCK2 in 1–6 h, and dramatically enhanced the expression of marker molecules on “stemness”, such as OCT4, SOX2, KLF4, MYC, NANOG, and CD133 in 6–72 h. However, all these changes in hepatocytes induced by −50 mmHg stimulation were almost abrogated by ROCK inhibitor Y27623. Our data suggest that an appropriate force of negative pressure stimulation can effectively induce the dedifferentiation of hepatocytes via RhoA/ROCK pathway activation. | |||||||||||||||||
言語 | en | |||||||||||||||||
書誌情報 |
en : Biochemical and Biophysical Research Communications 巻 667, p. 104-110, 発行日 2023-05-19 |
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出版者 | ||||||||||||||||||
言語 | en | |||||||||||||||||
出版者 | Elsevier B.V. | |||||||||||||||||
ISSN | ||||||||||||||||||
収録物識別子タイプ | ISSN | |||||||||||||||||
収録物識別子 | 0006291X | |||||||||||||||||
DOI | ||||||||||||||||||
関連タイプ | isIdenticalTo | |||||||||||||||||
識別子タイプ | DOI | |||||||||||||||||
関連識別子 | 10.1016/j.bbrc.2023.05.042 | |||||||||||||||||
権利 | ||||||||||||||||||
言語 | en | |||||||||||||||||
権利情報Resource | © 2023 Elsevier Inc. This manuscript version is made available under the CC-BY-NC-ND 4.0 license https://creativecommons.org/licenses/by-nc-nd/4.0/. | |||||||||||||||||
著者版フラグ | ||||||||||||||||||
出版タイプ | AM | |||||||||||||||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||||||
引用 | ||||||||||||||||||
内容記述タイプ | Other | |||||||||||||||||
内容記述 | Biochemical and Biophysical Research Communications, 667, pp.104-110; 2023 |