| アイテムタイプ |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2019-08-02 |
| タイトル |
|
|
タイトル |
Genome Editing of Babesia bovis Using the CRISPR/Cas9 System |
| 言語 |
|
|
言語 |
eng |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Babesia bovis |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
CRISPR/Cas9 |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
genome editing |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
thioredoxin peroxidase |
| 資源タイプ |
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|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| 著者 |
Hakimi, Hassan
Ishizaki, Takahiro
Kegawa, Yuto
Kaneko, Osamu
Kawazu, Shin-ichiro
Asada, Masahito
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Babesia bovis, the most virulent causative agent of bovine babesiosis, is prevalent in tropical and subtropical regions of the world. Although the whole-genome sequence was released more than a decade ago, functional analysis of the genomics of this parasite is hampered by the limited breadth of genetic engineering tools. In this study, we implemented the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system for B. bovis and demonstrated its potential for genome editing. Cas9 and human dihydrofolate reductase (hDHFR) were simultaneously expressed by the B. bovis elongation factor-1α bidirectional promoter, and a single guide RNA was expressed via the B. bovis U6 spliceosomal RNA promoter. Using a single plasmid construct, we were able to add an epitope tag to spherical body protein 3 (SBP3), introduce a point mutation into thioredoxin peroxidase 1 (tpx-1) to impair the function of the product, and replace the tpx-1 open reading frame with the other protein. Epitope tagging of SBP3 was efficient using this system, with a negligible number of remaining wild-type parasites and a pure transgenic population produced by allelic replacement of tpx-1. This advancement in genetic engineering tools for B. bovis will aid functional analysis of the genome and underpin characterization of candidate drug and vaccine targets. |
| 書誌情報 |
mSphere
巻 4,
号 3,
p. e00109-19,
発行日 2019-06-12
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| 出版者 |
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出版者 |
American Society for Microbiology |
| EISSN |
|
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収録物識別子タイプ |
ISSN |
|
収録物識別子 |
23795042 |
| DOI |
|
|
関連タイプ |
isIdenticalTo |
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|
識別子タイプ |
DOI |
|
|
関連識別子 |
10.1128/mSphere.00109-19 |
| 権利 |
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|
権利情報 |
c 2019 Hakimi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. |
| 著者版フラグ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| 引用 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
mSphere, 4(3), e00109-19; 2019 |
mSphere4_e00109-19.pdf (1.2 MB)
